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mutT 8-oxo-dGTP diphosphatase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944824, updated on 23-Nov-2023

Summary

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Gene symbol
mutT
Gene description
8-oxo-dGTP diphosphatase
Primary source
ASAP:ABE-0000346
Locus tag
b0099
See related
ECOCYC:EG10626
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0100; nudA
Summary
Nudix hydrolase. [More information is available at EcoGene: EG10626]. dGTP pyrophosphohydrolase (MutT) can catalyze the hydrolysis of all eight canonical nucleotides, but it shows a marked preference for dGTP. [More information is available at EcoCyc: EG10626].
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Genomic context

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Sequence:
NC_000913.3 (111044..111433)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene SecA translation regulator Neighboring gene protein translocation ATPase Neighboring gene DNA gyrase inhibitor YacG Neighboring gene cell division factor ZapD Neighboring gene dephospho-CoA kinase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Removal of 8-oxo-GTP by MutT hydrolase is not a major contributor to transcriptional fidelity. Gordon AJ, et al. Nucleic Acids Res, 2014 Oct 29. PMID 25294823, Free PMC Article
  2. Molecular actions of Escherichia coli MutT for control of spontaneous mutagenesis. Setoyama D, et al. Mutat Res, 2011 Feb 10. PMID 21147134
  3. Structural and dynamic features of the MutT protein in the recognition of nucleotides with the mutagenic 8-oxoguanine base. Nakamura T, et al. J Biol Chem, 2010 Jan 1. PMID 19864691, Free PMC Article
  4. Bacterial DNA repair genes and their eukaryotic homologues: 2. Role of bacterial mutator gene homologues in human disease. Overview of nucleotide pool sanitization and mismatch repair systems. Arczewska KD, et al. Acta Biochim Pol, 2007. PMID 17893750
  5. Transient state kinetic studies of the MutT-catalyzed nucleoside triphosphate pyrophosphohydrolase reaction. Xia Z, et al. Biochemistry, 2005 Nov 22. PMID 16285737

See all (53) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Degradation of the Escherichia coli Essential Proteins DapB and Dxr Results in Oxidative Stress, which Contributes to Lethality through Incomplete Base Excision Repair.
    Title: Degradation of the Escherichia coli Essential Proteins DapB and Dxr Results in Oxidative Stress, which Contributes to Lethality through Incomplete Base Excision Repair.
  2. The study investigated the role of MutT on transcription fidelity and find no increase in epigenetic switch frequency in the absence of MutT function.
    Title: Removal of 8-oxo-GTP by MutT hydrolase is not a major contributor to transcriptional fidelity.
  3. MutT mutation increased the point-mutation rate 150-fold.
    Title: Mutation rate dynamics in a bacterial population reflect tension between adaptation and genetic load.
  4. The MutT functions are not needed under anaerobic condition, yet the level of the MutT protein in cell is kept constant, probably for preparing for sudden changes of oxygen pressure.
    Title: Molecular actions of Escherichia coli MutT for control of spontaneous mutagenesis.
  5. The mutT defect does not elevate chromosomal fragmentation in Escherichia coli because of the surprisingly low levels of MutM/MutY-recognized DNA modifications.
    Title: The mutT defect does not elevate chromosomal fragmentation in Escherichia coli because of the surprisingly low levels of MutM/MutY-recognized DNA modifications.
  6. N-terminus verified by Edman degradation on complete protein
    Title: Studies on the mutator gene, mutT of Escherichia coli. Molecular cloning of the gene, purification of the gene product, and identification of a novel nucleoside triphosphatase.
  7. MutT hydrolyzes four types of 8-oxoGua (8-oxo-7,8-dihydroguanine)-containing nucleoside diphosphates and triphosphates to the related monophosphates, thereby preventing misincorporation of 8-oxoGua into DNA and RNA.
    Title: Multiple enzyme activities of Escherichia coli MutT protein for sanitization of DNA and RNA precursor pools.
  8. These results indicate that mutT plays important roles in mutagenesis induced by 2-OH-dATP and 8-OH-dGTP in vivo.
    Title: Mutagenesis by damaged deoxyribonucleotides and its prevention by MutT-type hydrolyzing enzymes.
  9. transient state kinetic analysis of the reaction pathway of mutT; findings confirm an iso kinetic mechanism and explain the preferred hydrolysis of 8-oxo-dGTP
    Title: Transient state kinetic studies of the MutT-catalyzed nucleoside triphosphate pyrophosphohydrolase reaction.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 8-oxo-7,8-dihydrodeoxyguanosine triphosphate pyrophosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 8-oxo-7,8-dihydrodeoxyguanosine triphosphate pyrophosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 8-oxo-7,8-dihydrodeoxyguanosine triphosphate pyrophosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables 8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables 8-oxo-GDP phosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 8-oxo-GDP phosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 8-oxo-GDP phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables 8-oxo-dGDP phosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 8-oxo-dGDP phosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 8-oxo-dGDP phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
NOT enables ATP diphosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables manganese ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA damage response IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA repair IC
Inferred by Curator
more info
 PubMed 
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in dGDP catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in dGTP catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in nucleotide-excision repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 

General protein information

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Preferred Names
8-oxo-dGTP diphosphatase
NP_414641.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    111044..111433
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414641.1 8-oxo-dGTP diphosphatase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414641.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8T6BEZ8
    Conserved Domains (1) summary
    PRK10776
    Location:1 → 129
    PRK10776; 8-oxo-dGTP diphosphatase MutT
Nucleotide Protein
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