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mhpC 2-hydroxy-6-ketonona-2,4-dienedioate hydrolase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944954, updated on 4-Apr-2024

Summary

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Gene symbol
mhpC
Gene description
2-hydroxy-6-ketonona-2,4-dienedioate hydrolase
Primary source
ASAP:ABE-0001201
Locus tag
b0349
See related
ECOCYC:M012
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0346
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Genomic context

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Sequence:
NC_000913.3 (371239..372105)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 3-(3-hydroxyphenyl)propanoate hydroxylase Neighboring gene 3-carboxyethylcatechol 2,3-dioxygenase Neighboring gene 2-hydroxypentadienoate hydratase Neighboring gene acetaldehyde dehydrogenase (acetylating) MhpF Neighboring gene 4-hydroxy-2-oxovalerate aldolase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Evidence for a gem-diol reaction intermediate in bacterial C-C hydrolase enzymes BphD and MhpC from 13C NMR spectroscopy. Li JJ, et al. Biochemistry, 2006 Oct 17. PMID 17029401
  2. Purification, characterization, and stereochemical analysis of a C-C hydrolase: 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase. Lam WW, et al. Biochemistry, 1997 Oct 7. PMID 9315862
  3. Overproduction, purification and properties of 2,3-dihydroxyphenylpropionate 1,2-dioxygenase from Escherichia coli. Bugg TD. Biochim Biophys Acta, 1993 Oct 6. PMID 8399388
  4. Catalytic role for arginine 188 in the C-C hydrolase catalytic mechanism for Escherichia coli MhpC and Burkholderia xenovorans LB400 BphD. Li C, et al. Biochemistry, 2006 Oct 17. PMID 17029402
  5. The structure of the C-C bond hydrolase MhpC provides insights into its catalytic mechanism. Dunn G, et al. J Mol Biol, 2005 Feb 11. PMID 15663942

See all (14) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. X ray structure
    Title: The structure of the C-C bond hydrolase MhpC provides insights into its catalytic mechanism.
  2. A catalytic mechanism is proposed involving stabilisation of reactive intermediates and activation of a nucleophilic water molecule by Ser110
    Title: Catalytic mechanism of C-C hydrolase MhpC from Escherichia coli: kinetic analysis of His263 and Ser110 site-directed mutants.
  3. Replacement of Arg-188 in MhpC with Gln and Lys led to 200- and 40-fold decreases, respectively, in k(cat).
    Title: Catalytic role for arginine 188 in the C-C hydrolase catalytic mechanism for Escherichia coli MhpC and Burkholderia xenovorans LB400 BphD.
  4. A new line-broadened signal was observed upon incubation of the (13)C-labeled substrate with an H114A MhpC mutant, which is able to accept the 6-phenyl-containing substrate, on a shorter time scale.
    Title: Evidence for a gem-diol reaction intermediate in bacterial C-C hydrolase enzymes BphD and MhpC from 13C NMR spectroscopy.
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Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 2-hydroxy-6-oxonona-2,4,7-trienedioate hydrolase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 2-hydroxy-6-oxonona-2,4,7-trienedioate hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within 3-(3-hydroxy)phenylpropionate catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in 3-phenylpropionate catabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in aromatic compound catabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within aromatic compound catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
2-hydroxy-6-ketonona-2,4-dienedioate hydrolase
NP_414883.5

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    371239..372105
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414883.5 2-hydroxy-6-ketonona-2,4-dienedioate hydrolase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414883.5

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    E1IZM4, E6BHH5
    Conserved Domains (1) summary
    TIGR03343
    Location:7 → 286
    biphenyl_bphD; 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

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Other Literature Sources
Molecular Biology Databases