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codA cytosine/isoguanine deaminase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944996, updated on 4-Apr-2024

Summary

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Gene symbol
codA
Gene description
cytosine/isoguanine deaminase
Primary source
ASAP:ABE-0001161
Locus tag
b0337
See related
ECOCYC:EG11326
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0334
Summary
Earlier characterization of the enzyme had found it to consist of two polypeptides of different molecular mass. [More information is available at EcoCyc: EG11326].
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Genomic context

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Sequence:
NC_000913.3 (356171..357454)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 2-methylcitrate dehydratase Neighboring gene propionyl-CoA synthetase Neighboring gene cytosine transporter Neighboring gene DNA-binding transcriptional dual regulator CynR Neighboring gene carbonic anhydrase 1

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Bacterial cytosine deaminase mutants created by molecular engineering show improved 5-fluorocytosine-mediated cell killing in vitro and in vivo. Fuchita M, et al. Cancer Res, 2009 Jun 1. PMID 19487291, Free PMC Article
  2. Procedure for the study of certain enzymes in minute amounts and its application to the investigation of cytosine deaminase. CHARGAFF E, et al. J Biol Chem, 1948 Sep. PMID 18880798
  3. Mutation of Escherichia coli cytosine deaminase significantly enhances molecular chemotherapy of human glioma. Kaliberov SA, et al. Gene Ther, 2007 Jul. PMID 17495948
  4. Protein transduction with bacterial cytosine deaminase fused to the TLM intercellular transport motif induces profound chemosensitivity to 5-fluorocytosine in human hepatoma cells. Hillemann A, et al. J Hepatol, 2005 Sep. PMID 15922474
  5. Random mutagenesis and selection of Escherichia coli cytosine deaminase for cancer gene therapy. Mahan SD, et al. Protein Eng Des Sel, 2004 Aug. PMID 15381761

See all (35) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that peptide deformylase, threonine dehydrogenase, and cytosine deaminase are very sensitve to hydrogen peroxide.
    Title: Mononuclear iron enzymes are primary targets of hydrogen peroxide stress.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: Characterization of the Escherichia coli codBA operon encoding cytosine permease and cytosine deaminase.
  3. Future therapeutic local application of cell-permeable cytosine deaminase-TLM fusion proteins together with a systemic fluorouracil prodrug application could result in profound hepatoma antitumor activities without apparent side effects.
    Title: Protein transduction with bacterial cytosine deaminase fused to the TLM intercellular transport motif induces profound chemosensitivity to 5-fluorocytosine in human hepatoma cells.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 5-fluorocytosine deaminase activity IEA
Inferred from Electronic Annotation
more info
  
enables cytosine deaminase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables cytosine deaminase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cytosine deaminase activity IEA
Inferred from Electronic Annotation
more info
  
enables ferrous iron binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables isoguanine deaminase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables isoguanine deaminase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in cytosine catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within cytosine catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cytosine metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol HDA PubMed 

General protein information

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Preferred Names
cytosine/isoguanine deaminase
NP_414871.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    356171..357454
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414871.1 cytosine/isoguanine deaminase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414871.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A2W6NWD8
    Conserved Domains (1) summary
    PRK09230
    Location:2 → 427
    PRK09230; cytosine deaminase; Provisional
Nucleotide Protein
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Gene LinkOut

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Other Literature Sources
Molecular Biology Databases
Research Materials