U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

mhpB 3-carboxyethylcatechol 2,3-dioxygenase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945047, updated on 4-Apr-2024

Summary

Go to the top of the page Help
Gene symbol
mhpB
Gene description
3-carboxyethylcatechol 2,3-dioxygenase
Primary source
ASAP:ABE-0001198
Locus tag
b0348
See related
ECOCYC:M011
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0345
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Sequence:
NC_000913.3 (370277..371221)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional activator MhpR Neighboring gene 3-(3-hydroxyphenyl)propanoate hydroxylase Neighboring gene 2-hydroxy-6-ketonona-2,4-dienedioate hydrolase Neighboring gene 2-hydroxypentadienoate hydratase Neighboring gene acetaldehyde dehydrogenase (acetylating) MhpF

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Evidence from mechanistic probes for distinct hydroperoxide rearrangement mechanisms in the intradiol and extradiol catechol dioxygenases. Xin M, et al. J Am Chem Soc, 2008 Aug 6. PMID 18627158
  2. Directed evolution of a non-heme-iron-dependent extradiol catechol dioxygenase: identification of mutants with intradiol oxidative cleavage activity. Schlosrich J, et al. Chembiochem, 2006 Dec. PMID 17051653
  3. Acid-base catalysis in the extradiol catechol dioxygenase reaction mechanism: site-directed mutagenesis of His-115 and His-179 in Escherichia coli 2,3-dihydroxyphenylpropionate 1,2-dioxygenase (MhpB). Mendel S, et al. Biochemistry, 2004 Oct 26. PMID 15491145
  4. Catechol dioxygenases from Escherichia coli (MhpB) and Alcaligenes eutrophus (MpcI): sequence analysis and biochemical properties of a third family of extradiol dioxygenases. Spence EL, et al. J Bacteriol, 1996 Sep. PMID 8752345, Free PMC Article
  5. Overproduction, purification and properties of 2,3-dihydroxyphenylpropionate 1,2-dioxygenase from Escherichia coli. Bugg TD. Biochim Biophys Acta, 1993 Oct 6. PMID 8399388

See all (13) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on complete protein
    Title: Overproduction, purification and properties of 2,3-dihydroxyphenylpropionate 1,2-dioxygenase from Escherichia coli.
  2. A catalytic mechanism involving His-179 and His-115 as acid-base catalytic residues is proposed for MhpB.
    Title: Acid-base catalysis in the extradiol catechol dioxygenase reaction mechanism: site-directed mutagenesis of His-115 and His-179 in Escherichia coli 2,3-dihydroxyphenylpropionate 1,2-dioxygenase (MhpB).
Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3-carboxyethylcatechol 2,3-dioxygenase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 3-carboxyethylcatechol 2,3-dioxygenase activity IEA
Inferred from Electronic Annotation
more info
  
enables dioxygenase activity IEA
Inferred from Electronic Annotation
more info
  
enables ferrous iron binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ferrous iron binding IEA
Inferred from Electronic Annotation
more info
  
enables iron ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within 3-(3-hydroxy)phenylpropionate catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in 3-phenylpropionate catabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in aromatic compound catabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in cellular aromatic compound metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within phenylpropanoid catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
3-carboxyethylcatechol 2,3-dioxygenase
NP_414882.1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    370277..371221
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414882.1 3-carboxyethylcatechol 2,3-dioxygenase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414882.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AWM6, A0A6C9QBU9, A0A8S7EJ18
    Conserved Domains (1) summary
    cd07365
    Location:1 → 311
    MhpB_like; Subunit B of the Class III Extradiol ring-cleavage dioxygenase, 2,3-dihydroxyphenylpropionate 1,2-dioxygenase (MhpB), which catalyzes the oxidization and subsequent ring-opening of 2,3-dihydroxyphenylpropionate
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.