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leuC 3-isopropylmalate dehydratase subunit LeuC [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945076, updated on 11-Apr-2024

Summary

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Gene symbol
leuC
Gene description
3-isopropylmalate dehydratase subunit LeuC
Primary source
ASAP:ABE-0000261
Locus tag
b0072
See related
ECOCYC:EG11576
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0074
Summary
Isopropylmalate isomerase catalyzes the second step in leucine biosynthesis, converting 2-isopropylmalate to 3-isopropylmalate. [More information is available at EcoCyc: EG11576].
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Genomic context

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Sequence:
NC_000913.3 (79464..80864, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene sugar exporter SetA Neighboring gene 3-isopropylmalate dehydratase subunit LeuD Neighboring gene 3-isopropylmalate dehydrogenase Neighboring gene 2-isopropylmalate synthase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Micromolar intracellular hydrogen peroxide disrupts metabolism by damaging iron-sulfur enzymes. Jang S, et al. J Biol Chem, 2007 Jan 12. PMID 17102132, Free PMC Article
  2. Hydrophobic interaction at the subunit interface contributes to the thermostability of 3-isopropylmalate dehydrogenase from an extreme thermophile, Thermus thermophilus. Kirino H, et al. Eur J Biochem, 1994 Feb 15. PMID 8119295
  3. Wild-type isopropylmalate isomerase in Salmonella typhimurium is composed of two different subunits. Fultz PN, et al. J Bacteriol, 1981 Oct. PMID 7026530, Free PMC Article
  4. Salmonella typhimurium newD and Escherichia coli leuC genes code for a functional isopropylmalate isomerase in Salmonella typhimurium-Escherichia coli hybrids. Fultz PN, et al. J Bacteriol, 1979 Mar. PMID 374346, Free PMC Article
  5. Genetic analysis of the leucine region in Escherichia coli B-r: gene-enzyme assignments. Yang HL, et al. J Bacteriol, 1974 Jan. PMID 4587614, Free PMC Article

See all (27) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. copper treatment rapidly inactivated isopropylmalate dehydratase
    Title: The iron-sulfur clusters of dehydratases are primary intracellular targets of copper toxicity.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: '98 Escherichia coli SWISS-2DPAGE database update.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3-isopropylmalate dehydratase activity IEA
Inferred from Electronic Annotation
more info
  
enables 4 iron, 4 sulfur cluster binding IC
Inferred by Curator
more info
 PubMed 
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in L-leucine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within L-leucine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in L-leucine biosynthetic process NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in branched-chain amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in carboxylic acid metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of 3-isopropylmalate dehydratase complex NAS
Non-traceable Author Statement
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
3-isopropylmalate dehydratase subunit LeuC
NP_414614.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    79464..80864 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414614.1 3-isopropylmalate dehydratase subunit LeuC [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414614.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8T6Q576
    Conserved Domains (1) summary
    PRK05478
    Location:1 → 466
    PRK05478; 3-isopropylmalate dehydratase large subunit
Nucleotide Protein
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Gene LinkOut

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Other Literature Sources
Molecular Biology Databases