acrA multidrug efflux pump membrane fusion lipoprotein AcrA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: acrA
Gene description: multidrug efflux pump membrane fusion lipoprotein AcrA
Primary source: ASAP:ABE-0001603
Locus tag: b0463
See related: ECOCYC:EG11703
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK0457; lir; Mb; mbl; mtcA; nbsA; sipB
Summary: acrA was first identified as an acridine sensitive mutation. acrA is a mutational suppressor of topA deletion inviability in low osmolarity medium. [More information is available at EcoGene: EG11703]. AcrA is the periplasmic lipoprotein component of the AcrAB-TolC multidrug efflux pump in Escherichia coli and can function in chimeric constructions with three other RND-family pumps, AcrD, AcrF and YhiV. [More information is available at EcoCyc: EG11703].
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Genomic context

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Sequence:: NC_000913.3 (484426..485619, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

In situ structure of the AcrAB-TolC efflux pump at subnanometer resolution.
Title: In situ structure of the AcrAB-TolC efflux pump at subnanometer resolution.
Pyridylpiperazine-based allosteric inhibitors of RND-type multidrug efflux pumps.
Title: Pyridylpiperazine-based allosteric inhibitors of RND-type multidrug efflux pumps.
Discovery of multidrug efflux pump inhibitors with a novel chemical scaffold.
Title: Discovery of multidrug efflux pump inhibitors with a novel chemical scaffold.
AcrA, the membrane fusion protein, connects the inner-membrane-bound AcrB to the outer-membrane-bound TolC to form the multidrug efflux pump.
Title: Conformational Dynamics of AcrA Govern Multidrug Efflux Pump Assembly.
In the presence of translation-inhibiting antibiotics, resistance acquisition depends on the AcrAB-TolC multidrug efflux pump, because it reduces tetracycline concentrations in the cell.
Title: Role of AcrAB-TolC multidrug efflux pump in drug-resistance acquisition by plasmid transfer.
It has been demonstrated that an intact AcrAB-TolC efflux pump is crucial to the development of bacterial quinolone resistance. Its activity is complemented by expression of the alternative AcrEF efflux pump.
Title: Upregulation of AcrEF in Quinolone Resistance Development in Escherichia coli When AcrAB-TolC Function Is Impaired.
Ten phosphorus ylides were compared in multidrug efflux pump system consisting of the subunits acridine-resistance proteins A and B (AcrA and AcrB) and the multidrug efflux pump outer membrane factor TolC (TolC) and in AcrAB-TolC-deletion.
Title: Fluorinated Beta-diketo Phosphorus Ylides Are Novel Efflux Pump Inhibitors in Bacteria.
Data show that CsrA binds directly to the 5' end of the transcript encoding AcrAB.
Title: CsrA maximizes expression of the AcrAB multidrug resistance transporter.
It has been found that both AcrA and AcrB lasted for approximately 6 days in live E. coli cells, and the stability of AcrB depended on the presence of AcrA but not on active antibiotic efflux.
Title: Study of the degradation of a multidrug transporter using a non-radioactive pulse chase method.
AdeABC was less effective than AcrAB-TolC in the extrusion of lipophilic beta-lactams, novobiocin, and ethidium bromide, although it was more effective at tetracycline efflux.
Title: Properties of AdeABC and AdeIJK efflux systems of Acinetobacter baumannii compared with those of the AcrAB-TolC system of Escherichia coli.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transmembrane transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in bile acid and bile salt transport	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within bile acid and bile salt transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to antibiotic	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to antibiotic	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to antibiotic	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to toxic substance	IEA Inferred from Electronic Annotation more info
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in xenobiotic detoxification by transmembrane export across the cell outer membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in xenobiotic detoxification by transmembrane export across the cell outer membrane	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within xenobiotic transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of efflux pump complex	IDA Inferred from Direct Assay more info	PubMed
part_of efflux pump complex	IPI Inferred from Physical Interaction more info	PubMed
part_of efflux pump complex	NAS Non-traceable Author Statement more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in outer membrane-bounded periplasmic space	IDA Inferred from Direct Assay more info	PubMed
located_in periplasmic side of plasma membrane	NAS Non-traceable Author Statement more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: multidrug efflux pump membrane fusion lipoprotein AcrA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

484426..485619 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_414996.1 multidrug efflux pump membrane fusion lipoprotein AcrA [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_414996.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A827EW84

Conserved Domains (1) summary

PRK15030
Location:1 → 397

PRK15030; multidrug efflux RND transporter periplasmic adaptor subunit AcrA