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thiM hydroxyethylthiazole kinase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945142, updated on 2-May-2024

Summary

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Gene symbol
thiM
Gene description
hydroxyethylthiazole kinase
Primary source
ASAP:ABE-0006960
Locus tag
b2104
See related
ECOCYC:M007
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2097
Summary
thiMD operon expression is controlled by a thiamine pyrophosphate (TPP) responsive riboswitch in the 5' UTR. [More information is available at EcoGene: EG14069]. Hydroxyethylthiazole kinase catalyzes one of two reactions that feed 4-methyl-5-(-hydroxyethyl)thiazole phosphate into the thiamine biosynthesis pathway. [More information is available at EcoCyc: M007].
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Genomic context

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Sequence:
NC_000913.3 (2184513..2185301, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative sugar kinase YegV Neighboring gene putative DNA-binding transcriptional regulator YegW Neighboring gene putative glycosyl hydrolase YegX Neighboring gene bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase Neighboring gene DNA-binding transcriptional repressor RcnR Neighboring gene Ni(2(+))/Co(2(+)) exporter

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Purification and properties of 4-methyl-5-hydroxyethylthiazole kinase from Escherichia coli. Tani Y, et al. Biosci Biotechnol Biochem, 2016. PMID 26634770
  2. Molecular basis of gene regulation by the THI-box riboswitch. Ontiveros-Palacios N, et al. Mol Microbiol, 2008 Feb. PMID 18179415
  3. Ligand-induced folding of the thiM TPP riboswitch investigated by a structure-based fluorescence spectroscopic approach. Lang K, et al. Nucleic Acids Res, 2007. PMID 17693433, Free PMC Article
  4. Conformational changes in the expression domain of the Escherichia coli thiM riboswitch. Rentmeister A, et al. Nucleic Acids Res, 2007. PMID 17517779, Free PMC Article
  5. Crystal structures of the thi-box riboswitch bound to thiamine pyrophosphate analogs reveal adaptive RNA-small molecule recognition. Edwards TE, et al. Structure, 2006 Sep. PMID 16962976

See all (21) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structural analysis of ligand-induced folding of a thiamine pyrophosphate responsive riboswitch from Escherichia coli thiM mRNA.
    Title: Ligand-induced folding of the thiM TPP riboswitch investigated by a structure-based fluorescence spectroscopic approach.
Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables hydroxyethylthiazole kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables hydroxyethylthiazole kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables prenol kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in thiamine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in thiamine diphosphate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within thiamine salvage IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
hydroxyethylthiazole kinase
NP_416607.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2184513..2185301 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416607.1 hydroxyethylthiazole kinase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416607.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8T6BZZ0
    Conserved Domains (1) summary
    pfam02110
    Location:15 → 259
    HK; Hydroxyethylthiazole kinase family
Nucleotide Protein
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