fepA ferric enterobactin outer membrane transporter [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: fepA
Gene description: ferric enterobactin outer membrane transporter
Primary source: ASAP:ABE-0002014
Locus tag: b0584
See related: ECOCYC:EG10293
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: cbr; cbt; ECK0576; fep; feuB
Summary: FepA is a protein involved with transport of enterobactin-iron across the outer membrane . [More information is available at EcoCyc: EG10293].
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Genomic context

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Sequence:: NC_000913.3 (610254..612494, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Colicin-Mediated Transport of DNA through the Iron Transporter FepA.
Title: Colicin-Mediated Transport of DNA through the Iron Transporter FepA.
Conformational rearrangements in the N-domain of Escherichia coli FepA during ferric enterobactin transport.
Title: Conformational rearrangements in the N-domain of Escherichia coli FepA during ferric enterobactin transport.
A stem-loop within E. coli fepA mRNA coding region activates translation initiation.
Title: Stem-Loop Structures within mRNA Coding Sequences Activate Translation Initiation and Mediate Control by Small Regulatory RNAs.
TonB and FepA display confined mobility within Escherichia coli cell and inner membranes.
Title: Confined Mobility of TonB and FepA in Escherichia coli Membranes.
The authors found numerous specific sites of FepA interaction with TonB on the periplasmic face of the FepA cork in addition to the TonB box.
Title: Going Outside the TonB Box: Identification of Novel FepA-TonB Interactions In Vivo.
When FepA binds to ferric enterobactin in outer bacterial membrane, it was reflected by conformational motion of the external vestibular loops.
Title: Concerted loop motion triggers induced fit of FepA to ferric enterobactin.
the E. coli FepA ortholog (EfeO) cannot replace FepA in FepB-driven iron release from heme indicating protein specificity in these activities
Title: Staphylococcus aureus FepA and FepB proteins drive heme iron utilization in Escherichia coli.
analysis of the uptake of ferric enterobactin (FeEnt) by Escherichia coli FepA
Title: Direct measurements of the outer membrane stage of ferric enterobactin transport: postuptake binding.
Modification of FepA by fluorescein maleimide was temperature-dependent, and was inhibited by colicin B binding to FepA.
Title: Fluoresceination of FepA during colicin B killing: effects of temperature, toxin and TonB.
N-terminus verified by Edman degradation on mature peptide
Title: Overproduction of the outer-membrane proteins FepA and FhuE responsible for iron transport in Escherichia coli hfq::cat mutant.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables colicin transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables colicin transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables colicin transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables enterobactin binding	IDA Inferred from Direct Assay more info	PubMed
enables enterobactin transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables ferric-enterobactin transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables ferric-enterobactin transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ligand-gated channel activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables siderophore uptake transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables siderophore-iron transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables signaling receptor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within colicin transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within colicin transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in enterobactin transport	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within ferric-enterobactin import into cell	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within ferric-enterobactin import into cell	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular iron ion homeostasis	NAS Non-traceable Author Statement more info	PubMed
involved_in iron ion transport	IEA Inferred from Electronic Annotation more info
involved_in monoatomic ion transport	IEA Inferred from Electronic Annotation more info
involved_in siderophore transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in siderophore transport	IEA Inferred from Electronic Annotation more info
involved_in siderophore-dependent iron import into cell	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within siderophore-dependent iron import into cell	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell envelope	IDA Inferred from Direct Assay more info	PubMed
is_active_in cell outer membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in cell outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cell outer membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	NAS Non-traceable Author Statement more info	PubMed
part_of transmembrane transporter complex	NAS Non-traceable Author Statement more info	PubMed

General protein information

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Preferred Names: ferric enterobactin outer membrane transporter

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

610254..612494 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_415116.1 ferric enterobactin outer membrane transporter [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_415116.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A7L5V372

Conserved Domains (1) summary

PRK13524
Location:1 → 746

PRK13524; FepA family TonB-dependent siderophore receptor