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mrdA peptidoglycan DD-transpeptidase MrdA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945240, updated on 11-Apr-2024

Summary

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Gene symbol
mrdA
Gene description
peptidoglycan DD-transpeptidase MrdA
Primary source
ASAP:ABE-0002178
Locus tag
b0635
See related
ECOCYC:EG10606
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0628; pbpA
Summary
Penicillin binding protein 2 (PBP2) is responsible for maintaining the rod cell shape and mecillinam sensitivity in Escherichia coli along with the product of the mrdB gene, RodA . mrdA and mrdB share a transcription unit . [More information is available at EcoCyc: EG10606].
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Genomic context

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Sequence:
NC_000913.3 (666316..668217, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene rare lipoprotein RlpA Neighboring gene peptidoglycan glycosyltransferase MrdB Neighboring gene 23S rRNA m(3)psi1915 methyltransferase Neighboring gene ribosomal silencing factor RsfS

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Identification of Mutations in the mrdA Gene Encoding PBP2 That Reduce Carbapenem and Diazabicyclooctane Susceptibility of Escherichia coli Clinical Isolates with Mutations in ftsI (PBP3) and Which Carry bla(NDM-1). Ranjitkar S, et al. mSphere, 2019 Jul 3. PMID 31270174, Free PMC Article
  2. Affinity of doripenem and comparators to penicillin-binding proteins in Escherichia coli and Pseudomonas aeruginosa. Davies TA, et al. Antimicrob Agents Chemother, 2008 Apr. PMID 18250190, Free PMC Article
  3. Polar positional information in Escherichia coli spherical cells. Pradel N, et al. Biochem Biophys Res Commun, 2007 Feb 9. PMID 17188233
  4. Bacterial cell shape-dependent inflammatory response in mammary epithelial cells. Khattar MM, et al. Curr Microbiol, 2006 Jun. PMID 16619114
  5. Target affinities of faropenem to and its impact on the morphology of gram-positive and gram-negative bacteria. Dalhoff A, et al. Chemotherapy, 2003 Jul. PMID 12886052

See all (85) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. mutations in PBP2 reduce susceptibility to carbapenems in E. coli clinical isolates that also have alterations of PBP3 and express the NDM-1 beta-lactamase
    Title: Identification of Mutations in the mrdA Gene Encoding PBP2 That Reduce Carbapenem and Diazabicyclooctane Susceptibility of Escherichia coli Clinical Isolates with Mutations in ftsI (PBP3) and Which Carry bla(NDM-1).
  2. Mutations in mreB, mrdA and mrdB suppress the shape defect of RodZ-deficient cells.
    Title: Mutations in cell elongation genes mreB, mrdA and mrdB suppress the shape defect of RodZ-deficient cells.
  3. Data show that MreB and FtsZ direct the synthesis of lateral cell wall via independent pathways that require PBP 2.
    Title: In Escherichia coli, MreB and FtsZ direct the synthesis of lateral cell wall via independent pathways that require PBP 2.
  4. Cells lacking mrdA were fully viable and propagated as small spheres under conditions of slow mass increase but formed large nondividing spheroids with noncanonical FtsZ assembly patterns at higher mass doubling rates.
    Title: Conditional lethality, division defects, membrane involution, and endocytosis in mre and mrd shape mutants of Escherichia coli.
  5. The morphological effects and lysis were also eliminated entirely when overproduction of penicillin-binding protein 2 (PBP 2) and variants was conducted.
    Title: Overproduction of penicillin-binding protein 2 and its inactive variants causes morphological changes and lysis in Escherichia coli.
Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables carboxypeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables penicillin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables penicillin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables penicillin binding IEA
Inferred from Electronic Annotation
more info
  
enables penicillin binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables peptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables peptidoglycan L,D-transpeptidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables peptidoglycan L,D-transpeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables serine-type D-Ala-D-Ala carboxypeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables serine-type D-Ala-D-Ala carboxypeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables serine-type D-Ala-D-Ala carboxypeptidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables serine-type D-Ala-D-Ala carboxypeptidase activity ISM
Inferred from Sequence Model
more info
 PubMed 
Process Evidence Code Pubs
involved_in cell wall organization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in cell wall organization IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cell wall organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within peptidoglycan biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in peptidoglycan biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within peptidoglycan biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in peptidoglycan biosynthetic process NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in proteolysis IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of cell shape IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within regulation of cell shape IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of cell shape NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within response to antibiotic IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in outer membrane-bounded periplasmic space ISM
Inferred from Sequence Model
more info
 PubMed 
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISM
Inferred from Sequence Model
more info
 PubMed 
located_in plasma membrane NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
peptidoglycan DD-transpeptidase MrdA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    666316..668217 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415168.1 peptidoglycan DD-transpeptidase MrdA [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415168.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A2W6PGZ2, A0A7A6VUS0
    Conserved Domains (1) summary
    PRK10795
    Location:1 → 632
    PRK10795; penicillin-binding protein 2; Provisional
Nucleotide Protein
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