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glnS glutamine--tRNA ligase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945310, updated on 2-May-2024

Summary

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Gene symbol
glnS
Gene description
glutamine--tRNA ligase
Primary source
ASAP:ABE-0002317
Locus tag
b0680
See related
ECOCYC:EG10390
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0668
Summary
Glutaminyl-tRNA synthetase (GlnRS) is a member of the family of aminoacyl tRNA synthetases, which interpret the genetic code by covalently linking amino acids to their specific tRNA molecules. [More information is available at EcoCyc: EG10390].
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Genomic context

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Sequence:
NC_000913.3 (706093..707757)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene glucosamine-6-phosphate deaminase Neighboring gene N-acetylglucosamine-specific PTS enzyme IIABC component Neighboring gene ncRNA Neighboring gene chitooligosaccharide outer membrane channel

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Preliminary X-ray crystallographic analysis of an engineered glutamyl-tRNA synthetase from Escherichia coli. Chongdar N, et al. Acta Crystallogr F Struct Biol Commun, 2014 Jul. PMID 25005090, Free PMC Article
  2. Architectural underpinnings of the genetic code for glutamine. Corigliano EM, et al. Biochemistry, 2009 Feb 3. PMID 19128026, Free PMC Article
  3. A rationally engineered misacylating aminoacyl-tRNA synthetase. Bullock TL, et al. Proc Natl Acad Sci U S A, 2008 May 27. PMID 18477696, Free PMC Article
  4. Mechanism of the difference in the binding affinity of E. coli tRNAGln to glutaminyl-tRNA synthetase caused by noninterface nucleotides in variable loop. Yamasaki S, et al. Biophys J, 2007 Jan 1. PMID 17028132, Free PMC Article
  5. Active-site assembly in glutaminyl-tRNA synthetase by tRNA-mediated induced fit. Uter NT, et al. Biochemistry, 2006 Jun 6. PMID 16734422, Free PMC Article

See all (82) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The designed double mutant (K236E/E328A) of glutamyl-tRNA synthetase has been successfully crystallized.
    Title: Preliminary X-ray crystallographic analysis of an engineered glutamyl-tRNA synthetase from Escherichia coli.
  2. E. coli glutamyl-tRNA synthetase is inhibited by anticodon stem-loop domains and a minihelix
    Title: E. coli glutamyl-tRNA synthetase is inhibited by anticodon stem-loop domains and a minihelix.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
  4. the sequence difference in the variable loop of glutaminyl-tRNA synthetase causes the difference in the internal mobility of the tertiary core region tRNAs and leads to the difference in the affinity to aminoacyl-tRNA synthetases through the entropy term
    Title: Mechanism of the difference in the binding affinity of E. coli tRNAGln to glutaminyl-tRNA synthetase caused by noninterface nucleotides in variable loop.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables aminoacyl-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables glutamine-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glutamine-tRNA ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glutamine-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in glutaminyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within glutaminyl-tRNA aminoacylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in glutaminyl-tRNA aminoacylation IEA
Inferred from Electronic Annotation
more info
  
involved_in glutamyl-tRNA aminoacylation IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA aminoacylation for protein translation IEA
Inferred from Electronic Annotation
more info
  
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
glutamine--tRNA ligase
NP_415206.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    706093..707757
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415206.1 glutamine--tRNA ligase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415206.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7JXG0, A0A8S7R4V6, A0A8S7TQQ7
    Conserved Domains (1) summary
    PRK05347
    Location:1 → 552
    PRK05347; glutaminyl-tRNA synthetase; Provisional
Nucleotide Protein
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Gene LinkOut

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Other Literature Sources
Molecular Biology Databases
Research Materials