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metK methionine adenosyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945389, updated on 4-Apr-2024

Summary

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Gene symbol
metK
Gene description
methionine adenosyltransferase
Primary source
ASAP:ABE-0009650
Locus tag
b2942
See related
ECOCYC:EG10589
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2937; metX
Summary
The adjacent ORF yqgD has been removed from EcoGene because it is very unlikely to be a gene; the limited conservation with Salmonella is apparently due to a pseudotranslation of the conserved metK CRP site, promoter and the metK 5' UTR. [More information is available at EcoGene: EG10589]. Methionine adenosyltransferase catalyzes the formation of the sulfonium compound S-adenosylmethionine. [More information is available at EcoCyc: EG10589].
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Genomic context

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Sequence:
NC_000913.3 (3086706..3087860)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YqgG Neighboring gene protein YqgC Neighboring gene DUF2684 domain-containing protein YqgD Neighboring gene galactose:H(+) symporter Neighboring gene protein YggI

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Conversion of a chaperonin GroEL-independent protein into an obligate substrate. Ishimoto T, et al. J Biol Chem, 2014 Nov 14. PMID 25288795, Free PMC Article
  2. Discovery of novel types of inhibitors of S-adenosylmethionine synthesis by virtual screening. Taylor JC, et al. J Med Chem, 2009 Oct 8. PMID 19739644, Free PMC Article
  3. An investigation of the catalytic mechanism of S-adenosylmethionine synthetase by QM/MM calculations. Markham GD, et al. Arch Biochem Biophys, 2009 Dec. PMID 19699176, Free PMC Article
  4. In vivo hydrolysis of S-adenosyl-L-methionine in Escherichia coli increases export of 5-methylthioribose. Hughes JA. Can J Microbiol, 2006 Jun. PMID 16788729
  5. Crystal structure of the S-adenosylmethionine synthetase ternary complex: a novel catalytic mechanism of S-adenosylmethionine synthesis from ATP and Met. Komoto J, et al. Biochemistry, 2004 Feb 24. PMID 14967023

See all (64) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. analysis of key features that define the GroE dependence on MetK proteins
    Title: Conversion of a chaperonin GroEL-independent protein into an obligate substrate.
  2. The metK deficient Escherichia coli mutant uses externally provided S-adenosylmethionine as methyl donor to produce most but not all of its methionine, by methylation of homocysteine catalysed by MmuM.
    Title: Cell division, one-carbon metabolism and methionine synthesis in a metK-deficient Escherichia coli mutant, and a role for MmuM.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: The cadmium-stress stimulon of Escherichia coli K-12.
  4. Data suggest that a deficiency in S-adenosylmethionine synthetase/S-adenosylmethionine stops cell division at a specific point in the septation process.
    Title: A deficiency in S-adenosylmethionine synthetase interrupts assembly of the septal ring in Escherichia coli K-12.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables methionine adenosyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methionine adenosyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables methionine adenosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables potassium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables potassium ion binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in S-adenosylmethionine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in S-adenosylmethionine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within S-adenosylmethionine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in S-adenosylmethionine cycle IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in one-carbon metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
methionine adenosyltransferase
NP_417417.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3086706..3087860
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417417.1 methionine adenosyltransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417417.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A485JD64
    Conserved Domains (1) summary
    PRK05250
    Location:3 → 383
    PRK05250; S-adenosylmethionine synthetase; Validated
Nucleotide Protein
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