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mdfA multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945448, updated on 11-Apr-2024

Summary

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Gene symbol
mdfA
Gene description
multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
Primary source
ASAP:ABE-0002871
Locus tag
b0842
See related
ECOCYC:G6440
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
cmlA; cmr; ECK0832
Summary
Major Facilitator Superfamily (MFS). [More information is available at EcoGene: EG13696]. The MdfA protein, also known as Cmr, is a multidrug efflux protein belonging to the major facilitator superfamily (MFS) . [More information is available at EcoCyc: G6440].
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Genomic context

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Sequence:
NC_000913.3 (883673..884905)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional repressor DeoR Neighboring gene undecaprenyl pyrophosphate phosphatase Neighboring gene uncharacterized protein YbjH Neighboring gene 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase Neighboring gene inner membrane protein YbjJ

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Enhanced internal ionic interaction of MFS efflux pump MdfA contributes to its elevated antibiotic export. Li Y, et al. Phys Chem Chem Phys, 2022 Dec 21. PMID 36510750
  2. Probing the solution structure of the E. coli multidrug transporter MdfA using DEER distance measurements with nitroxide and Gd(III) spin labels. Yardeni EH, et al. Sci Rep, 2019 Aug 29. PMID 31467343, Free PMC Article
  3. Structure of an engineered multidrug transporter MdfA reveals the molecular basis for substrate recognition. Wu HH, et al. Commun Biol, 2019. PMID 31240248, Free PMC Article
  4. Outward open conformation of a Major Facilitator Superfamily multidrug/H(+) antiporter provides insights into switching mechanism. Nagarathinam K, et al. Nat Commun, 2018 Oct 1. PMID 30275448, Free PMC Article
  5. The fascinating but mysterious mechanistic aspects of multidrug transport by MdfA from Escherichia coli. Yardeni EH, et al. Res Microbiol, 2018 Sep-Oct. PMID 28951231

See all (55) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Enhanced internal ionic interaction of MFS efflux pump MdfA contributes to its elevated antibiotic export.
    Title: Enhanced internal ionic interaction of MFS efflux pump MdfA contributes to its elevated antibiotic export.
  2. Probing the solution structure of the E. coli multidrug transporter MdfA using DEER distance measurements with nitroxide and Gd(III) spin labels.
    Title: Probing the solution structure of the E. coli multidrug transporter MdfA using DEER distance measurements with nitroxide and Gd(III) spin labels.
  3. analysis of MdfA variant I239T/G354E in complexes with three electrically different ligands reveals the molecular basis for substrate recognition
    Title: Structure of an engineered multidrug transporter MdfA reveals the molecular basis for substrate recognition.
  4. Outward open conformation of the Major Facilitator Superfamily multidrug/H(+) antiporter MdfA from Escherichia coli has changes in relative orientations of the N- and C-terminal lobes, and transmembrane helix5 (TM5) is kinked and twisted. In vitro reconstitution experiments demonstrate the importance of selected residues for transport and molecular dynamics simulations provide insights into antiporter switching.
    Title: Outward open conformation of a Major Facilitator Superfamily multidrug/H(+) antiporter provides insights into switching mechanism.
  5. MdfA has exceptional promiscuous capabilities not only in its interaction with a plethora of chemically dissimilar compounds, but also concerning the various faces of its modes of energy consumption. [review]
    Title: The fascinating but mysterious mechanistic aspects of multidrug transport by MdfA from Escherichia coli.
  6. Crystallization and X-ray diffraction analysis of the multidrug-resistance transporter MdfA from Escherichia coli has been reported.
    Title: The multidrug-resistance transporter MdfA from Escherichia coli: crystallization and X-ray diffraction analysis.
  7. The MdfA structure consists of four pseudo-symmetrical, three-helix repeats, consistent with the typical structure of an MFS transporter.
    Title: Substrate-bound structure of the E. coli multidrug resistance transporter MdfA.
  8. The mdfA gene can function as transporter responsible for multidrug resistance, and also mediated the synergistic function with its surrounding genes in conjugative plasmids.
    Title: Structural and environmental features of novel mdfA variant and mdfA genes in recombinant regions of Escherichia coli.
  9. GlpG is able to recognize and cleave truncated forms of MdfA but not the intact protein.
    Title: Cleavage of a multispanning membrane protein by an intramembrane serine protease.
  10. the Mdr-binding pocket of MdfA is conformationally sensitive
    Title: A promiscuous conformational switch in the secondary multidrug transporter MdfA.
Submit: New GeneRIF Correction See all GeneRIFs (15)

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables potassium:proton antiporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables sodium:proton antiporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables sodium:proton antiporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in potassium ion transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in proton transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within regulation of cellular pH IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to antibiotic IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to antibiotic IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in sodium ion transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in xenobiotic detoxification by transmembrane export across the plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within xenobiotic detoxification by transmembrane export across the plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within xenobiotic detoxification by transmembrane export across the plasma membrane IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISM
Inferred from Sequence Model
more info
 PubMed 

General protein information

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Preferred Names
multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    883673..884905
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415363.1 multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415363.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0AA87G721
    Conserved Domains (1) summary
    PRK15402
    Location:7 → 410
    PRK15402; MdfA family multidrug efflux MFS transporter
Nucleotide Protein
Heading Accession and Version

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