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folC bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945451, updated on 2-May-2024

Summary

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Gene symbol
folC
Gene description
bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase
Primary source
ASAP:ABE-0007645
Locus tag
b2315
See related
ECOCYC:EG10327
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
dedC; ECK2309
Summary
folC codes for a bifunctional polypeptide. [More information is available at EcoCyc: EG10327].
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Genomic context

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Sequence:
NC_000913.3 (2431674..2432942, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene amidophosphoribosyltransferase Neighboring gene colicin V production protein Neighboring gene cell division protein DedD Neighboring gene acetyl-CoA carboxyltransferase subunit beta Neighboring gene DedA family protein DedA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A domino effect in antifolate drug action in Escherichia coli. Kwon YK, et al. Nat Chem Biol, 2008 Oct. PMID 18724364, Free PMC Article
  2. Mutagenesis of folylpolyglutamate synthetase indicates that dihydropteroate and tetrahydrofolate bind to the same site. Sheng Y, et al. Biochemistry, 2008 Feb 26. PMID 18232714
  3. Escherichia coli FolC structure reveals an unexpected dihydrofolate binding site providing an attractive target for anti-microbial therapy. Mathieu M, et al. J Biol Chem, 2005 May 13. PMID 15705579
  4. A simple radioassay for dihydrofolate synthetase activity in Escherichia coli and its application to an inhibition study of new pteroate analogs. Ho RI. Methods Enzymol, 1980. PMID 6768963
  5. Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase. Cloning and high expression of the Escherichia coli folC gene and purification and properties of the gene product. Bognar AL, et al. J Biol Chem, 1985 May 10. PMID 2985605

See all (21) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The uridylyltransferase GlnD and tRNA modification GTPase MnmE allosterically control Escherichia coli folylpoly-gamma-glutamate synthase FolC.
    Title: The uridylyltransferase GlnD and tRNA modification GTPase MnmE allosterically control Escherichia coli folylpoly-γ-glutamate synthase FolC.
  2. mutagenesis study to identify structural elements of folylpolyglutamate synthetase that are directly responsible for the functional differences between the E. coli and the Lactobacillus casei enzymes
    Title: Mutagenesis of folylpolyglutamate synthetase indicates that dihydropteroate and tetrahydrofolate bind to the same site.
  3. N-terminus verified by Edman degradation on complete protein
    Title: Primary structure of the Escherichia coli folC gene and its folylpolyglutamate synthetase-dihydrofolate synthetase product and regulation of expression by an upstream gene.
  4. analysis of an unexpected dihydrofolate binding site in Escherichia coli FolC
    Title: Escherichia coli FolC structure reveals an unexpected dihydrofolate binding site providing an attractive target for anti-microbial therapy.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables acid-amino acid ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables dihydrofolate synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables dihydrofolate synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables dihydrofolate synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables guanosine tetraphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables tetrahydrofolylpolyglutamate synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables tetrahydrofolylpolyglutamate synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables tetrahydrofolylpolyglutamate synthase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in dihydrofolate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in folic acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in folic acid-containing compound biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in folic acid-containing compound biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in one-carbon metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in tetrahydrofolate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in tetrahydrofolylpolyglutamate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase
NP_416818.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2431674..2432942 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416818.1 bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416818.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0AA87I3D1
    Conserved Domains (1) summary
    PRK10846
    Location:1 → 416
    PRK10846; bifunctional folylpolyglutamate synthase/ dihydrofolate synthase; Provisional
Nucleotide Protein
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