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murJ lipid II flippase MurJ [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945487, updated on 11-Apr-2024

Summary

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Gene symbol
murJ
Gene description
lipid II flippase MurJ
Primary source
ASAP:ABE-0003630
Locus tag
b1069
See related
ECOCYC:G6561
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1054; mviN; yceN
Summary
murJ(yceN) is an essental gene and conditional lethal mutants sweel and burst and acumulate the murein intermediate polyprenyl diphosphate-N-acetylmuramic acid-(pentapeptide)-N-acetyl-glucosamine at the non-permissive temperature (Inoue, 2008). murJ(yceN) was identified as one of two ORFs adjacent to the flg locus in a region originally named mviS, then renamed mviN and mviM when sequenced; mviMN was thought to be required for mouse virulence in Salmonella, but the mviMN deletion in ST39 also removed flgM (Carsiotis, 1989). flgM was later shown to be able to complement the mviS phenotype, and thus flgM is the real mouse virulence gene in this region of the Salmonella chromosome not mviS (Schmitt, 1994). [More information is available at EcoGene: EG13880]. MurJ is an essential gene involved in murein synthesis as the flippase responsible for transport of lipid II from the inner face of the inner membrane to the outer face for polymerization . [More information is available at EcoCyc: G6561].
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Genomic context

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Sequence:
NC_000913.3 (1127839..1129374)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF480 domain-containing protein YceH Neighboring gene putative oxidoreductase YceM Neighboring gene flagellar biosynthesis protein FlgN Neighboring gene anti-sigma factor for FliA (sigma(28))

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Crystal structure of the lipid flippase MurJ in a "squeezed" form distinct from its inward- and outward-facing forms. Kohga H, et al. Structure, 2022 Aug 4. PMID 35660157
  2. The bacterial lipid II flippase MurJ functions by an alternating-access mechanism. Kumar S, et al. J Biol Chem, 2019 Jan 18. PMID 30482840, Free PMC Article
  3. FtsW activity and lipid II synthesis are required for recruitment of MurJ to midcell during cell division in Escherichia coli. Liu X, et al. Mol Microbiol, 2018 Sep. PMID 30112777
  4. Structure and mutagenic analysis of the lipid II flippase MurJ from Escherichia coli. Zheng S, et al. Proc Natl Acad Sci U S A, 2018 Jun 26. PMID 29891673, Free PMC Article
  5. Direct observation of the influence of cardiolipin and antibiotics on lipid II binding to MurJ. Bolla JR, et al. Nat Chem, 2018 Mar. PMID 29461535, Free PMC Article

See all (26) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Crystal structure of the lipid flippase MurJ in a ""squeezed"" form distinct from its inward- and outward-facing forms.
    Title: Crystal structure of the lipid flippase MurJ in a "squeezed" form distinct from its inward- and outward-facing forms.
  2. study provides in vivo evidence that MurJ uses an alternating-access mechanism during the lipid II transport cycle.
    Title: The bacterial lipid II flippase MurJ functions by an alternating-access mechanism.
  3. MurJ localizes both in the lateral membrane and at midcell, and is recruited to midcell simultaneously with late-localizing divisome proteins and proteins MraY and MurG. This study visualizes MurJ localization in vivo and reveals a possible mechanism of MurJ recruitment during cell division.
    Title: FtsW activity and lipid II synthesis are required for recruitment of MurJ to midcell during cell division in Escherichia coli.
  4. The crystal structure of substrate-free MurJ reveals an inward-open conformation. High-throughput mutagenesis and next-generation sequencing assessed mutational tolerance at every amino acid in the protein, providing a detailed functional and structural map for the enzyme and identifying sites for inhibitor development. A rocker-switch model for lipid II transport was supported.
    Title: Structure and mutagenic analysis of the lipid II flippase MurJ from Escherichia coli.
  5. From these data, authors hypothesize that some charged residues in the cavity region of MurJ homologs are required for interaction with lipid II and/or energy coupling during transport.
    Title: Charge requirements of lipid II flippase activity in Escherichia coli.
  6. MurJ is the lipid II flippase in Escherichia coli.
    Title: Bacterial cell wall. MurJ is the flippase of lipid-linked precursors for peptidoglycan biogenesis.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables cardiolipin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables lipid-linked peptidoglycan transporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables lipid-linked peptidoglycan transporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables lipid-linked peptidoglycan transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables lipid-linked peptidoglycan transporter activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in cell wall organization IEA
Inferred from Electronic Annotation
more info
  
involved_in glycolipid translocation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in lipid translocation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in lipid-linked peptidoglycan transport IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within lipid-linked peptidoglycan transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in lipid-linked peptidoglycan transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within lipid-linked peptidoglycan transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in peptidoglycan biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in peptidoglycan biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within peptidoglycan biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of cell shape IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in division septum IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 

General protein information

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Preferred Names
lipid II flippase MurJ

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1127839..1129374
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415587.1 lipid II flippase MurJ [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415587.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376W583
    Conserved Domains (1) summary
    COG0728
    Location:1 → 510
    MviN; Peptidoglycan biosynthesis protein MviN/MurJ, putative lipid II flippase [Cell wall/membrane/envelope biogenesis]
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

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