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focA formate channel FocA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945513, updated on 11-Apr-2024

Summary

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Gene symbol
focA
Gene description
formate channel FocA
Primary source
ASAP:ABE-0003073
Locus tag
b0904
See related
ECOCYC:EG11258
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0895; ycaE
Summary
FocA is a membrane protein with six TM regions. [More information is available at EcoGene: EG11258]. FocA is a putative formate transporter, possibly involved in both formate uptake and efflux. [More information is available at EcoCyc: EG11258].
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Genomic context

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Sequence:
NC_000913.3 (953609..954466, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene pyruvate formate-lyase activating enzyme Neighboring gene pyruvate formate-lyase Neighboring gene ribosomal protein S12 methylthiotransferase accessory factor YcaO Neighboring gene DUF421 domain-containing protein YcaP

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Distinguishing functional from structural roles of conserved pore residues during formate translocation by the FocA anion channel. Kammel M, et al. Microbiologyopen, 2022 Aug. PMID 36031960, Free PMC Article
  2. Interplay between the Conserved Pore Residues Thr-91 and His-209 Controls Formate Translocation through the FocA Channel. Kammel M, et al. Microb Physiol, 2022. PMID 35390794
  3. Anaerobic Formate and Hydrogen Metabolism. Pinske C, et al. EcoSal Plus, 2016 Oct. PMID 27735784
  4. Pyruvate formate-lyase interacts directly with the formate channel FocA to regulate formate translocation. Doberenz C, et al. J Mol Biol, 2014 Jul 29. PMID 24887098, Free PMC Article
  5. Identification of key residues in the formate channel FocA that control import and export of formate. Hunger D, et al. Biol Chem, 2014 Jul. PMID 24659605

See all (30) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Distinguishing functional from structural roles of conserved pore residues during formate translocation by the FocA anion channel.
    Title: Distinguishing functional from structural roles of conserved pore residues during formate translocation by the FocA anion channel.
  2. Interplay between the Conserved Pore Residues Thr-91 and His-209 Controls Formate Translocation through the FocA Channel.
    Title: Interplay between the Conserved Pore Residues Thr-91 and His-209 Controls Formate Translocation through the FocA Channel.
  3. Our results suggest that regulation of formate translocation direction by FocA/FocB channels is important for maintaining internal pH and proton motive force by modulating H2 production.
    Title: pH and a mixed carbon-substrate spectrum influence FocA- and FocB-dependent, formate-driven H2 production in Escherichia coli.
  4. in vivo competitive assays showed that one of the second steps (focA) exhibited positive epistasis with the first, while another (dcuB) exhibited negative epistasis. The data shows that strong effect adaptive mutations continuously recur in gut commensal bacterial species.
    Title: A Mutational Hotspot and Strong Selection Contribute to the Order of Mutations Selected for during Escherichia coli Adaptation to the Gut.
  5. Highly conserved amino acid residues within FocA identified in structural studies are predicted to be important in the control of formate translocation.
    Title: Identification of key residues in the formate channel FocA that control import and export of formate.
  6. FocA has a strong preference for formate as a substrate in vivo and not other acidic fermentation products and the tight coupling between FocA and PflB synthesis ensures adequate substrate delivery to the appropriate formate dehydrogenase.
    Title: Coordination of FocA and pyruvate formate-lyase synthesis in Escherichia coli demonstrates preferential translocation of formate over other mixed-acid fermentation products.
  7. Analysis of the oligomeric state by blue-native polyacrylamide gel electrophoresis revealed FocA to have an unexpected pentameric quaternary structure.
    Title: Unexpected oligomeric structure of the FocA formate channel of Escherichia coli : a paradigm for the formate-nitrite transporter family of integral membrane proteins.
  8. Deletion of the E. coli focA gene (together with deletions of other ferementation disruption genes) increased production of succinate
    Title: Eliminating side products and increasing succinate yields in engineered strains of Escherichia coli C.
Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables formate transmembrane transporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables formate transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables formate transmembrane transporter activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in formate transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in formate transport IEA
Inferred from Electronic Annotation
more info
  
involved_in formate transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mixed acid fermentation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to acidic pH TAS
Traceable Author Statement
more info
 PubMed 
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISM
Inferred from Sequence Model
more info
 PubMed 

General protein information

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Preferred Names
formate channel FocA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    953609..954466 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415424.1 formate channel FocA [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415424.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AD34
    Conserved Domains (1) summary
    PRK10805
    Location:1 → 285
    PRK10805; formate transporter; Provisional
Nucleotide Protein
Heading Accession and Version

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