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aroA 3-phosphoshikimate 1-carboxyvinyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945528, updated on 2-May-2024

Summary

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Gene symbol
aroA
Gene description
3-phosphoshikimate 1-carboxyvinyltransferase
Primary source
ASAP:ABE-0003095
Locus tag
b0908
See related
ECOCYC:EG10073
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0899
Summary
[More information is available at EcoCyc: EG10073].
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Genomic context

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Sequence:
NC_000913.3 (958812..960095)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF421 domain-containing protein YcaP Neighboring gene phosphoserine/phosphohydroxythreonine aminotransferase Neighboring gene periplasmic protease YcaL Neighboring gene cytidylate kinase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants. Cao G, et al. PLoS One, 2012. PMID 22715408, Free PMC Article
  2. Acid-induced formation of molten globule states in the wild type Escherichia coli 5-enolpyruvylshikimate 3-phosphate synthase and its three mutated forms: G96A, A183T and G96A/A183T. Haghani K, et al. Protein J, 2011 Feb. PMID 21327882
  3. A novel RPMXR motif among class II 5-enolpyruvylshikimate-3-phosphate synthases is required for enzymatic activity and glyphosate resistance. Li L, et al. J Biotechnol, 2009 Dec. PMID 19799945
  4. Catalytic residues and an electrostatic sandwich that promote enolpyruvyl shikimate 3-phosphate synthase (AroA) catalysis. Berti PJ, et al. Biochemistry, 2009 May 5. PMID 19271774
  5. Structural basis of glyphosate resistance resulting from the double mutation Thr97 -> Ile and Pro101 -> Ser in 5-enolpyruvylshikimate-3-phosphate synthase from Escherichia coli. Funke T, et al. J Biol Chem, 2009 Apr 10. PMID 19211556, Free PMC Article

See all (56) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants.
    Title: A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants.
  2. The results suggest that there is a direct relationship between stability of 5-enolpyruvylshikimate 3-phosphate synthase and pH stability of its folding intermediates
    Title: Acid-induced formation of molten globule states in the wild type Escherichia coli 5-enolpyruvylshikimate 3-phosphate synthase and its three mutated forms: G96A, A183T and G96A/A183T.
  3. We identified a novel RPMXR motif that is strictly conserved among class II EPSP synthases and is required for enzymatic acitvity and glyphosate resistance.
    Title: A novel RPMXR motif among class II 5-enolpyruvylshikimate-3-phosphate synthases is required for enzymatic activity and glyphosate resistance.
  4. In an active site that is overwhelmingly positively charged, Asp-313 and Glu-341 side chains of aroA form an "electrostatic sandwich" around the positive charge at C2 in cationic intermediates/transition states, stabilizing them and promoting catalysis.
    Title: Catalytic residues and an electrostatic sandwich that promote enolpyruvyl shikimate 3-phosphate synthase (AroA) catalysis.
  5. analysis of glyphosate resistance resulting from the double mutation Thr97 -> Ile and Pro101 -> Ser in 5-enolpyruvylshikimate-3-phosphate synthase from Escherichia coli
    Title: Structural basis of glyphosate resistance resulting from the double mutation Thr97 -> Ile and Pro101 -> Ser in 5-enolpyruvylshikimate-3-phosphate synthase from Escherichia coli.
  6. glyphosate ternary complexes of P101S and P101L 5-enolpyruvylshikimate-3-phosphate synthase were determined to between 1.5- and 1.6-A resolution
    Title: Structural basis of glyphosate tolerance resulting from mutations of Pro101 in Escherichia coli 5-enolpyruvylshikimate-3-phosphate synthase.
Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3-phosphoshikimate 1-carboxyvinyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 3-phosphoshikimate 1-carboxyvinyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 3-phosphoshikimate 1-carboxyvinyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in aromatic amino acid family biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in chorismate biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within chorismate biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in chorismate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
3-phosphoshikimate 1-carboxyvinyltransferase
NP_415428.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    958812..960095
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415428.1 3-phosphoshikimate 1-carboxyvinyltransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415428.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    Q7WVX4
    Conserved Domains (1) summary
    cd01554
    Location:12 → 423
    EPT-like; Enol pyruvate transferases family includes EPSP synthases and UDP-N-acetylglucosamine enolpyruvyl transferase. Both enzymes catalyze the reaction of enolpyruvyl transfer.
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

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Other Literature Sources
Molecular Biology Databases
Research Materials