U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

ompF outer membrane porin F [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945554, updated on 11-Apr-2024

Summary

Go to the top of the page Help
Gene symbol
ompF
Gene description
outer membrane porin F
Primary source
ASAP:ABE-0003153
Locus tag
b0929
See related
ECOCYC:EG10671
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
cmlB; cry; ECK0920; nfxB; tolF
Summary
Trimeric. [More information is available at EcoGene: EG10671]. OmpF is a member of the General Bacterial Porin (GBP) family. [More information is available at EcoCyc: EG10671].
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Sequence:
NC_000913.3 (985894..986982, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene hydroxyacylglutathione hydrolase GloC Neighboring gene aspartate aminotransferase Neighboring gene asparagine--tRNA ligase Neighboring gene nicotinate phosphoribosyltransferase

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Designer Outer Membrane Protein Facilitates Uptake of Decoy Molecules into a Cytochrome P450BM3-Based Whole-Cell Biocatalyst. Karasawa M, et al. Angew Chem Int Ed Engl, 2022 Feb 7. PMID 34704327
  2. Porin threading drives receptor disengagement and establishes active colicin transport through Escherichia coli OmpF. Francis MR, et al. EMBO J, 2021 Nov 2. PMID 34515361, Free PMC Article
  3. OmpF Downregulation Mediated by Sigma E or OmpR Activation Confers Cefalexin Resistance in Escherichia coli in the Absence of Acquired β-Lactamases. Alzayn M, et al. Antimicrob Agents Chemother, 2021 Oct 18. PMID 34460299, Free PMC Article
  4. A protocol for production of perdeuterated OmpF porin for neutron crystallography. Aggarwal S, et al. Protein Expr Purif, 2021 Dec. PMID 34416360
  5. Lipid binding attenuates channel closure of the outer membrane protein OmpF. Liko I, et al. Proc Natl Acad Sci U S A, 2018 Jun 26. PMID 29891712, Free PMC Article

See all (177) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Study of the early response of Escherichia coli lpcA and ompF mutants to ciprofloxacin.
    Title: Study of the early response of Escherichia coli lpcA and ompF mutants to ciprofloxacin.
  2. Designer Outer Membrane Protein Facilitates Uptake of Decoy Molecules into a Cytochrome P450BM3-Based Whole-Cell Biocatalyst.
    Title: Designer Outer Membrane Protein Facilitates Uptake of Decoy Molecules into a Cytochrome P450BM3-Based Whole-Cell Biocatalyst.
  3. A protocol for production of perdeuterated OmpF porin for neutron crystallography.
    Title: A protocol for production of perdeuterated OmpF porin for neutron crystallography.
  4. Porin threading drives receptor disengagement and establishes active colicin transport through Escherichia coli OmpF.
    Title: Porin threading drives receptor disengagement and establishes active colicin transport through Escherichia coli OmpF.
  5. OmpF Downregulation Mediated by Sigma E or OmpR Activation Confers Cefalexin Resistance in Escherichia coli in the Absence of Acquired beta-Lactamases.
    Title: OmpF Downregulation Mediated by Sigma E or OmpR Activation Confers Cefalexin Resistance in Escherichia coli in the Absence of Acquired β-Lactamases.
  6. Trimeric porins, such as ompF, have specific lipopolysaccharide binding sites that are essential for porin biogenesis.
    Title: Gram-negative trimeric porins have specific LPS binding sites that are essential for porin biogenesis.
  7. Klebsiella pneumoniae OmpK35 and OmpK36 produced larger more permeable channels than their Escherichia coli homologs OmpF and OmpC.
    Title: Klebsiella pneumoniae Major Porins OmpK35 and OmpK36 Allow More Efficient Diffusion of β-Lactams than Their Escherichia coli Homologs OmpF and OmpC.
  8. The results suggest that trimerization is crucial for both structure and function of general porin OmpF in E. coli, whereas being trimer in substrate-specific channel OprP supports a pore function in Pseudomonas aeruginosa.
    Title: Why do the outer membrane proteins OmpF from E. coli and OprP from P. aeruginosa prefer trimers? Simulation studies.
  9. Two different centered monoclinic crystals of the E. coli outer-membrane protein OmpF originate from the same building block
    Title: Two different centered monoclinic crystals of the E. coli outer-membrane protein OmpF originate from the same building block.
  10. The site of lipopolysaccharide binding means that ColN will preferably bind at the interface and thus position itself close to the surface of its translocon component, OmpF.
    Title: The antibacterial toxin colicin N binds to the inner core of lipopolysaccharide and close to its translocator protein.
Submit: New GeneRIF Correction See all GeneRIFs (26)

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables colicin transmembrane transporter activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables disordered domain specific binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables lipid binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables lipopolysaccharide binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables monoatomic ion channel activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables porin activity IEA
Inferred from Electronic Annotation
more info
  
enables porin activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables porin activity TAS
Traceable Author Statement
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in bacteriocin transport IEA
Inferred from Electronic Annotation
more info
  
involved_in monoatomic ion transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in monoatomic ion transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in monoatomic ion transmembrane transport TAS
Traceable Author Statement
more info
 PubMed 
involved_in monoatomic ion transport IEA
Inferred from Electronic Annotation
more info
  
involved_in protein homotrimerization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cell outer membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cell outer membrane IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
part_of monoatomic ion channel complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of pore complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of pore complex IEA
Inferred from Electronic Annotation
more info
  

General protein information

Go to the top of the page Help
Preferred Names
outer membrane porin F

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    985894..986982 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415449.1 outer membrane porin F [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415449.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    C9E721
    Conserved Domains (1) summary
    PRK10002
    Location:1 → 362
    PRK10002; porin OmpF
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.