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fabA beta-hydroxyacyl-acyl carrier protein dehydratase/isomerase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945568, updated on 4-Feb-2024

Summary

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Gene symbol
fabA
Gene description
beta-hydroxyacyl-acyl carrier protein dehydratase/isomerase
Primary source
ASAP:ABE-0003229
Locus tag
b0954
See related
ECOCYC:EG10273
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0945
Summary
Binds TrxA (Kumar, 2004). [More information is available at EcoGene: EG10273].
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Genomic context

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Sequence:
NC_000913.3 (1015952..1016470, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene intermembrane transport protein PqiB Neighboring gene intermembrane transport lipoprotein PqiC Neighboring gene ribosome modulation factor Neighboring gene putative ATP-dependent protease YcbZ Neighboring gene macrodomain Ter protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Control of Unsaturation in De Novo Fatty Acid Biosynthesis by FabA. Bartholow TG, et al. Biochemistry, 2022 Apr 5. PMID 35255690, Free PMC Article
  2. Complex binding of the FabR repressor of bacterial unsaturated fatty acid biosynthesis to its cognate promoters. Feng Y, et al. Mol Microbiol, 2011 Apr. PMID 21276098, Free PMC Article
  3. Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation. Luo LH, et al. Biotechnol Lett, 2009 Dec. PMID 19685209
  4. Expression of the Escherichia coli fabA gene encoding beta-hydroxydecanoyl thioester dehydrase and transport to chloroplasts in transgenic tobacco. Saito K, et al. Transgenic Res, 1995 Jan. PMID 7881463
  5. Beta-hydroxydecanoyl thio ester dehydrase does not catalyze a rate-limiting step in Escherichia coli unsaturated fatty acid synthesis. Clark DP, et al. Biochemistry, 1983 Dec 6. PMID 6362720

See all (44) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Control of Unsaturation in De Novo Fatty Acid Biosynthesis by FabA.
    Title: Control of Unsaturation in De Novo Fatty Acid Biosynthesis by FabA.
  2. The FabA is a soluble protein that has been relatively straightforward to express and to purify from cultured E. coli, FabZ has shown to be mostly insoluble and only partially active.
    Title: Fused dimerization increases expression, solubility, and activity of bacterial dehydratase enzymes.
  3. The studies highlight key interactions of transient ACP-FabA protein complexes and define the dynamic movements of ACP that facilitate substrate delivery and trigger complex dissociation.
    Title: Crystal structures of acyl carrier protein in complex with two catalytic partners show a dynamic role in cellular metabolism.
  4. Data indicate that despite the remarkably similar arrangements of promoter elements, FadR predominately regulates fabA expression whereas FabR is the dominant regulator of fabB expression.
    Title: Complex binding of the FabR repressor of bacterial unsaturated fatty acid biosynthesis to its cognate promoters.
  5. Recombinant E. coli harboring fabA had elevated tolerance against ethanol compared to wild type strain.
    Title: Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation.
  6. the key reaction in UFA synthesis catalyzed by FabB is elongation of the cis-3-decenoyl-ACP produced by FabA.
    Title: Escherichia coli unsaturated fatty acid synthesis: complex transcription of the fabA gene and in vivo identification of the essential reaction catalyzed by FabB.
  7. N-terminus verified by Edman degradation on complete protein
    Title: Derived amino acid sequence and identification of active site residues of Escherichia coli beta-hydroxydecanoyl thioester dehydrase.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables (3R)-3-hydroxybutanoyl-[acyl-carrier-protein] hydratase activity IEA
Inferred from Electronic Annotation
more info
  
enables (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity IEA
Inferred from Electronic Annotation
more info
  
enables (3R)-3-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity IEA
Inferred from Electronic Annotation
more info
  
enables (3R)-3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables (3R)-3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity IEA
Inferred from Electronic Annotation
more info
  
enables (3R)-3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity IEA
Inferred from Electronic Annotation
more info
  
enables (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity IEA
Inferred from Electronic Annotation
more info
  
enables isomerase activity IEA
Inferred from Electronic Annotation
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables trans-2-decenoyl-acyl-carrier-protein isomerase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables trans-2-decenoyl-acyl-carrier-protein isomerase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in fatty acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within fatty acid biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in fatty acid metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in lipid metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
beta-hydroxyacyl-acyl carrier protein dehydratase/isomerase
NP_415474.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1015952..1016470 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415474.1 beta-hydroxyacyl-acyl carrier protein dehydratase/isomerase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415474.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A828AK35
    Conserved Domains (1) summary
    PRK05174
    Location:1 → 172
    PRK05174; bifunctional 3-hydroxydecanoyl-ACP dehydratase/trans-2-decenoyl-ACP isomerase
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

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Other Literature Sources
Molecular Biology Databases
Research Materials