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mgsA methylglyoxal synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945574, updated on 23-Nov-2023

Summary

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Gene symbol
mgsA
Gene description
methylglyoxal synthase
Primary source
ASAP:ABE-0003259
Locus tag
b0963
See related
ECOCYC:G6497
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0954; mgs; yccG
Summary
Methylglyoxal synthase (MGS) catalyzes the apparently irreversible formation of methylglyoxal from glycerone phosphate. [More information is available at EcoCyc: G6497].
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Genomic context

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Sequence:
NC_000913.3 (1026557..1027015, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene PF03733 family inner membrane protein YccF Neighboring gene DNA helicase IV Neighboring gene DUF2057 domain-containing protein YccT Neighboring gene putative HspQ acetyl donor

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Deletion of methylglyoxal synthase gene (mgsA) increased sugar co-metabolism in ethanol-producing Escherichia coli. Yomano LP, et al. Biotechnol Lett, 2009 Sep. PMID 19458924, Free PMC Article
  2. Mutagenic studies on histidine 98 of methylglyoxal synthase: effects on mechanism and conformational change. Marks GT, et al. Biochemistry, 2004 Apr 6. PMID 15049687
  3. The regulation of Escherichia coli methylglyoxal synthase; a new control site in glycolysis? Hopper DJ, et al. FEBS Lett, 1971 Mar 16. PMID 11945670
  4. Mechanistic implications of methylglyoxal synthase complexed with phosphoglycolohydroxamic acid as observed by X-ray crystallography and NMR spectroscopy. Marks GT, et al. Biochemistry, 2001 Jun 12. PMID 11389594
  5. Mirroring perfection: the structure of methylglyoxal synthase complexed with the competitive inhibitor 2-phosphoglycolate. Saadat D, et al. Biochemistry, 2000 Mar 21. PMID 10715115

See all (31) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Deletion of the mgsA gene (strain LY168) resulted in the co-metabolism of glucose and xylose, and accelerated the metabolism of a 5-sugar mixture (mannose, glucose, arabinose, xylose and galactose) to ethanol.
    Title: Deletion of methylglyoxal synthase gene (mgsA) increased sugar co-metabolism in ethanol-producing Escherichia coli.
  2. Deletion of the E. coli mgsA gene (together with deletions of other ferementation disruption genes) increased production of succinate
    Title: Eliminating side products and increasing succinate yields in engineered strains of Escherichia coli C.
  3. N-terminus verified by Edman degradation on complete protein
    Title: Identification of catalytic bases in the active site of Escherichia coli methylglyoxal synthase: cloning, expression, and functional characterization of conserved aspartic acid residues.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables methylglyoxal synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methylglyoxal synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables methylglyoxal synthase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in methylglyoxal biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in methylglyoxal biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within methylglyoxal biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within protein hexamerization IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
methylglyoxal synthase
NP_415483.2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1026557..1027015 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415483.2 methylglyoxal synthase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415483.2

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A7L7E8H2
    Conserved Domains (1) summary
    PRK05234
    Location:8 → 148
    mgsA; methylglyoxal synthase; Validated
Nucleotide Protein
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Gene LinkOut

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