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pyrC dihydroorotase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945787, updated on 11-Apr-2024

Summary

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Gene symbol
pyrC
Gene description
dihydroorotase
Primary source
ASAP:ABE-0003607
Locus tag
b1062
See related
ECOCYC:EG10806
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1047
Summary
Dihydroorotase catalyzes the cyclization of carbamoyl-aspartate to dihydroorotate. [More information is available at EcoCyc: EG10806].
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Genomic context

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Sequence:
NC_000913.3 (1121561..1122607, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene regulator of biofilm formation Neighboring gene DNA damage-inducible protein I Neighboring gene putative lipid-binding lipoprotein YceB Neighboring gene reduced glutaredoxin 2

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Kinetic and structural analysis of mutant Escherichia coli dihydroorotases: a flexible loop stabilizes the transition state. Lee M, et al. Biochemistry, 2007 Sep 18. PMID 17711307
  2. Structures of ligand-free and inhibitor complexes of dihydroorotase from Escherichia coli: implications for loop movement in inhibitor design. Lee M, et al. J Mol Biol, 2007 Jul 27. PMID 17550785
  3. Structure of the T109S mutant of Escherichia coli dihydroorotase complexed with the inhibitor 5-fluoroorotate: catalytic activity is reflected by the crystal form. Lee M, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2007 Mar 1. PMID 17329804, Free PMC Article
  4. Inhibitors designed for the active site of dihydroorotase. Li Y, et al. Bioorg Chem, 2005 Dec. PMID 16213543
  5. Dihydroorotase from Escherichia coli: loop movement and cooperativity between subunits. Lee M, et al. J Mol Biol, 2005 May 6. PMID 15826651

See all (40) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. DAta show that expression of chromosomal dihydroorotase activity and beta-galactosidase activity of pyrC-lacZ fusion plasmid was repressed to about 30% and 17%, respectively in the fur+ strain compared to those in the fur- strain.
    Title: Effect of fur on pyrC gene expression.
  2. we report the structures of Escherichia coli DHOase crystallized without ligand (1.7 A resolution) and in the presence of the inhibitors 2-oxo-1,2,3,6-tetrahydropyrimidine-4,6-dicarboxylate (2.0 A) and 5-fluoroorotate (2.2 A).
    Title: Structures of ligand-free and inhibitor complexes of dihydroorotase from Escherichia coli: implications for loop movement in inhibitor design.
  3. The kinetic and structural analyses suggest that Thr109 and Thr110 in the flexible loop provide productive binding of substrate and stabilize the transition-state intermediate, thereby increasing catalytic activity.
    Title: Kinetic and structural analysis of mutant Escherichia coli dihydroorotases: a flexible loop stabilizes the transition state.
  4. N-terminus verified by Edman degradation on mature peptide
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
  5. Results show that a loop comprised of residues 105-115 of dihydroorotase has different conformations in its two subunits.
    Title: Dihydroorotase from Escherichia coli: loop movement and cooperativity between subunits.
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Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables dihydroorotase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables dihydroorotase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables dihydroorotase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in 'de novo' UMP biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in 'de novo' pyrimidine nucleobase biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within 'de novo' pyrimidine nucleobase biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in pyrimidine nucleobase biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in pyrimidine nucleotide biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in pyrimidine nucleotide biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
dihydroorotase
NP_415580.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1121561..1122607 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415580.1 dihydroorotase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415580.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9A9P6, A0A827Y1Q6
    Conserved Domains (1) summary
    PRK05451
    Location:4 → 347
    PRK05451; dihydroorotase; Provisional
Nucleotide Protein
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