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tonB Ton complex subunit TonB [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945843, updated on 4-Apr-2024

Summary

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Gene symbol
tonB
Gene description
Ton complex subunit TonB
Primary source
ASAP:ABE-0004196
Locus tag
b1252
See related
ECOCYC:EG11012
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1246; exbA; T1rec
Summary
Periplasmic TonB is attached to the cytoplasmic membrane by a signal anchor and interacts with outer membrane proteins, thus TonB spans the periplasmic space. [More information is available at EcoGene: EG11012]. TonB is a cytoplasmic membrane protein which transduces the proton motive force (pmf) of the cytoplasmic membrane to the outer membrane active transporters thus providing the energy source required for the import of iron-siderophore complexes and vitamin B12 across the outer membrane . [More information is available at EcoCyc: EG11012].
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Genomic context

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Sequence:
NC_000913.3 (1311089..1311808)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene K(+) channel Kch Neighboring gene protein YciI Neighboring gene acyl-CoA thioesterase YciA Neighboring gene inner membrane protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. From Homodimer to Heterodimer and Back: Elucidating the TonB Energy Transduction Cycle. Gresock MG, et al. J Bacteriol, 2015 Nov. PMID 26283773, Free PMC Article
  2. Avian pathogenic Escherichia coli ΔtonB mutants are safe and protective live-attenuated vaccine candidates. Holden KM, et al. Vet Microbiol, 2014 Oct 10. PMID 25205199
  3. Monomeric TonB and the Ton box are required for the formation of a high-affinity transporter-TonB complex. Freed DM, et al. Biochemistry, 2013 Apr 16. PMID 23517233, Free PMC Article
  4. TonB is essential for virulence in avian pathogenic Escherichia coli. Holden KM, et al. Comp Immunol Microbiol Infect Dis, 2012 Mar. PMID 22237011
  5. Taking the Escherichia coli TonB transmembrane domain "offline"? Nonprotonatable Asn substitutes fully for TonB His20. Swayne C, et al. J Bacteriol, 2011 Aug. PMID 21665976, Free PMC Article

See all (128) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. HDX-MS performed on BtuB in E. coli outer membranes delineates the luminal domain's allostery and unfolding upon B12 and TonB binding.
    Title: HDX-MS performed on BtuB in E. coli outer membranes delineates the luminal domain's allostery and unfolding upon B12 and TonB binding.
  2. TonB and FepA display confined mobility within Escherichia coli cell and inner membranes.
    Title: Confined Mobility of TonB and FepA in Escherichia coli Membranes.
  3. The authors found numerous specific sites of FepA interaction with TonB on the periplasmic face of the FepA cork in addition to the TonB box.
    Title: Going Outside the TonB Box: Identification of Novel FepA-TonB Interactions In Vivo.
  4. The TonB system embodies a novel means of active transport across the outer membrane for nutrients.
    Title: From Homodimer to Heterodimer and Back: Elucidating the TonB Energy Transduction Cycle.
  5. ExbB-ExbD-TonB form complex in molar ratios of 4:1:1.
    Title: Membrane Protein Complex ExbB4-ExbD1-TonB1 from Escherichia coli Demonstrates Conformational Plasticity.
  6. Avian pathogenic Escherichia coli DeltatonB mutants are safe and protective live-attenuated vaccine candidates.
    Title: Avian pathogenic Escherichia coli ΔtonB mutants are safe and protective live-attenuated vaccine candidates.
  7. This study has demonstrated that TonB is essential for virulence in avian pathogenic Escherichia coli.
    Title: TonB is essential for virulence in avian pathogenic Escherichia coli.
  8. These observations supported the hypothesis that ExbD couples TonB to the protonmotive force, with concomitant transitions of ExbD and TonB periplasmic domains from unenergized to energized heterodimers.
    Title: ExbD mutants define initial stages in TonB energization.
  9. ExbD-TonB interactions required ExbD transmembrane domain residue D25.
    Title: The same periplasmic ExbD residues mediate in vivo interactions between ExbD homodimers and ExbD-TonB heterodimers.
  10. ExbB(6) and ExbB(6)ExbD(1) are subcomplexes on which the final complex including TonB is assembled
    Title: Oligomeric structure of ExbB and ExbB-ExbD isolated from Escherichia coli as revealed by LILBID mass spectrometry.
Submit: New GeneRIF Correction See all GeneRIFs (29)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables energy transducer activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables energy transducer activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables energy transducer activity IEA
Inferred from Electronic Annotation
more info
  
enables energy transducer activity TAS
Traceable Author Statement
more info
 PubMed 
enables protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein domain specific binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in bacteriocin transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cobalamin transport IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within cobalamin transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cobalamin transport NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within colicin transport TAS
Traceable Author Statement
more info
 PubMed 
involved_in intracellular iron ion homeostasis NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in intracellular monoatomic cation homeostasis NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within receptor-mediated bacteriophage irreversible attachment to host cell TAS
Traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within siderophore transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in siderophore transport IEA
Inferred from Electronic Annotation
more info
  
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cell envelope IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cell outer membrane NAS
Non-traceable Author Statement
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in membrane NAS
Non-traceable Author Statement
more info
 PubMed 
located_in outer membrane-bounded periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in outer membrane-bounded periplasmic space IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
part_of plasma membrane protein complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of plasma membrane protein complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of transmembrane transporter complex NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
Ton complex subunit TonB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1311089..1311808
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415768.1 Ton complex subunit TonB [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415768.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A0A0FQP4, A0A1X3JK35, V0AQS9
    Conserved Domains (1) summary
    PRK10819
    Location:1 → 239
    PRK10819; transport protein TonB; Provisional
Nucleotide Protein
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