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uidA beta-glucuronidase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946149, updated on 2-May-2024

Summary

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Gene symbol
uidA
Gene description
beta-glucuronidase
Primary source
ASAP:ABE-0005410
Locus tag
b1617
See related
ECOCYC:EG11055
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1612; gurA; gusA
Summary
UidA has an Ig-like domain: pfam PF00703 aligns to residues 182-272. ebgA, uidA and lacZ are paralogs. [More information is available at EcoGene: EG11055]. D-glucuronidase catalyzes the cleavage of a wide variety of -glucuronides . -D-glucuronidase and |FRAME: [More information is available at EcoCyc: EG11055].
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Genomic context

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Sequence:
NC_000913.3 (1694260..1696071, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF945 domain-containing protein YdgA Neighboring gene outer membrane porin family protein UidC Neighboring gene glucuronide:H(+) symporter Neighboring gene DNA-binding transcriptional repressor UidR Neighboring gene 7-alpha-hydroxysteroid dehydrogenase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Drug-Encoded Biomarkers for Monitoring Biological Therapies. Tsoneva D, et al. PLoS One, 2015. PMID 26348361, Free PMC Article
  2. Native Electrophoresis-Coupled Activity Assays Reveal Catalytically-Active Protein Aggregates of Escherichia coli β-Glucuronidase. Burchett GG, et al. PLoS One, 2015. PMID 26121040, Free PMC Article
  3. Alleviating cancer drug toxicity by inhibiting a bacterial enzyme. Wallace BD, et al. Science, 2010 Nov 5. PMID 21051639, Free PMC Article
  4. Synthesis of all stereoisomers of 3-hydroxypipecolic acid and 3-hydroxy-4,5-dehydropipecolic acid and their evaluation as glycosidase inhibitors. Ohara C, et al. Bioorg Med Chem Lett, 2008 Mar 15. PMID 18296050
  5. Concurrent mutations in six amino acids in beta-glucuronidase improve its thermostability. Xiong AS, et al. Protein Eng Des Sel, 2007 Jul. PMID 17557766

See all (41) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. We found GusA to be the most reliable biomarker for monitoring oncolytic virotherapy among the three tested markers
    Title: Drug-Encoded Biomarkers for Monitoring Biological Therapies.
  2. there is not only an active dimer of the E. coli form of beta-glucuronidase, but also several larger active complexes, including an octomer and a 16-mer
    Title: Native Electrophoresis-Coupled Activity Assays Reveal Catalytically-Active Protein Aggregates of Escherichia coli β-Glucuronidase.
  3. we performed in vitro directed evolution & obtained a mutant GUS that was highly thermostable. mutation & reversion approaches identified 6 key AAs (Q493R, T509A, M532T, N550S, G559S and N566S) that are responsible for the improved thermostability
    Title: Concurrent mutations in six amino acids in beta-glucuronidase improve its thermostability.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables beta-glucuronidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables beta-glucuronidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables beta-glucuronidase activity IEA
Inferred from Electronic Annotation
more info
  
enables carbohydrate binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables hydrolase activity, acting on glycosyl bonds IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, hydrolyzing O-glycosyl compounds IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in carbohydrate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in glucuronoside catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within glucuronoside catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
beta-glucuronidase
NP_416134.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1694260..1696071 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416134.1 beta-glucuronidase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416134.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8I0CCI6
    Conserved Domains (1) summary
    PRK10150
    Location:1 → 600
    PRK10150; beta-D-glucuronidase; Provisional
Nucleotide Protein
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