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metC cystathionine beta-lyase/L-cysteine desulfhydrase/alanine racemase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946240, updated on 4-Apr-2024

Summary

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Gene symbol
metC
Gene description
cystathionine beta-lyase/L-cysteine desulfhydrase/alanine racemase
Primary source
ASAP:ABE-0009878
Locus tag
b3008
See related
ECOCYC:EG10583
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3000
Summary
The Flint et al. [More information is available at EcoGene: EG10583]. Several distinct proteins exhibit cysteine desulfhydrase activity, which is involved in cysteine degradation . [More information is available at EcoCyc: EG10583].
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Genomic context

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Sequence:
NC_000913.3 (3152236..3153423)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene Ton complex subunit ExbD Neighboring gene Ton complex subunit ExbB Neighboring gene DedA family protein YghB Neighboring gene DNA-binding transcriptional activator YqhC

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Cystathionine β-lyase is involved in d-amino acid metabolism. Miyamoto T, et al. Biochem J, 2018 Apr 23. PMID 29592871
  2. Characterization of the side-chain hydroxyl moieties of residues Y56, Y111, Y238, Y338, and S339 as determinants of specificity in E. coli cystathionine β-lyase. Lodha PH, et al. Biochemistry, 2011 Nov 15. PMID 21958132
  3. REPRESSION BY METHIONINE OF CYSTATHIONASE FORMATION IN ESCHERICHIA COLI. ROWBURY RJ, et al. J Gen Microbiol, 1964 Apr. PMID 14171258
  4. Slow-binding inhibition of Escherichia coli cystathionine beta-lyase by L-aminoethoxyvinylglycine: a kinetic and X-ray study. Clausen T, et al. Biochemistry, 1997 Oct 14. PMID 9376370
  5. Mode of action of cystathionine beta-lyase. Clausen T, et al. Biol Chem, 1997 Mar-Apr. PMID 9165088

See all (28) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. a representative of fold type III, the Escherichia coli alanine racemase (ALR), is a promiscuous cystathionine beta-lyase (CBL). Furthermore, E. coli CBL (fold type I) is a promiscuous alanine racemase. A single round of error-prone PCR and selection yielded variant ALR(Y274F), which catalyzes cystathionine beta-elimination with a near-native Michaelis constant (Km = 3.3 mm) but a poor turnover number (kcat approximate...
    Title: Mechanistic and Evolutionary Insights from the Reciprocal Promiscuity of Two Pyridoxal Phosphate-dependent Enzymes.
  2. A series of site-directed variants of CBL was constructed to investigate the roles of the hydroxyl moieties of active-site residues Y56, Y111, Y238, Y338, and S339 as determinants of specificity.
    Title: Characterization of the side-chain hydroxyl moieties of residues Y56, Y111, Y238, Y338, and S339 as determinants of specificity in E. coli cystathionine β-lyase.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: Studies on the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase in escherichia coli crude extract. Isolation of O-acetylserine sulfhydrylases A and B and beta-cystathionase based on their ability to mobilize sulfur from cysteine and to participate in Fe-S cluster synthesis.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables L-cysteine desulfhydrase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables L-cysteine desulfhydrase activity IEA
Inferred from Electronic Annotation
more info
  
enables L-cysteine desulfhydrase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables alanine racemase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables cystathionine beta-lyase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables cystathionine beta-lyase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cystathionine beta-lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables cysteine-S-conjugate beta-lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables pyridoxal phosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxal phosphate binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in L-cysteine catabolic process to pyruvate IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within L-cysteine catabolic process to pyruvate IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in amino acid metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in methionine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within methionine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within protein homotetramerization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in transsulfuration IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
cystathionine beta-lyase/L-cysteine desulfhydrase/alanine racemase
NP_417481.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3152236..3153423
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417481.1 cystathionine beta-lyase/L-cysteine desulfhydrase/alanine racemase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417481.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6N8NBX4, A0A8S7ECS9
    Conserved Domains (1) summary
    PRK08114
    Location:1 → 395
    PRK08114; cystathionine beta-lyase; Provisional
Nucleotide Protein
Heading Accession and Version

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