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lptE lipopolysaccharide assembly protein LptE [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946257, updated on 23-Nov-2023

Summary

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Gene symbol
lptE
Gene description
lipopolysaccharide assembly protein LptE
Primary source
ASAP:ABE-0002194
Locus tag
b0641
See related
ECOCYC:EG10855
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0634; rlpB
Summary
First 18 aa are a type II signal peptide. [More information is available at EcoGene: EG10855]. LptE (formally RlpB) is involved with lipopolysaccharide assembly in the outer membrane as part of the outer membrane LPS assembly complex with Imp . [More information is available at EcoCyc: EG10855].
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Genomic context

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Sequence:
NC_000913.3 (671605..672186, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative adenosylcobalamin phosphatase/alpha-ribazole phosphatase Neighboring gene nicotinate-nucleotide adenylyltransferase Neighboring gene DNA polymerase III subunit delta Neighboring gene leucine--tRNA ligase Neighboring gene DUF1451 domain-containing protein YbeL

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. LptE binds to and alters the physical state of LPS to catalyze its assembly at the cell surface. Malojčić G, et al. Proc Natl Acad Sci U S A, 2014 Jul 1. PMID 24938785, Free PMC Article
  2. Dominant negative lptE mutation that supports a role for LptE as a plug in the LptD barrel. Grabowicz M, et al. J Bacteriol, 2013 Mar. PMID 23316047, Free PMC Article
  3. The complex that inserts lipopolysaccharide into the bacterial outer membrane forms a two-protein plug-and-barrel. Freinkman E, et al. Proc Natl Acad Sci U S A, 2011 Feb 8. PMID 21257904, Free PMC Article
  4. Nonconsecutive disulfide bond formation in an essential integral outer membrane protein. Ruiz N, et al. Proc Natl Acad Sci U S A, 2010 Jul 6. PMID 20566849, Free PMC Article
  5. Characterization of the two-protein complex in Escherichia coli responsible for lipopolysaccharide assembly at the outer membrane. Chng SS, et al. Proc Natl Acad Sci U S A, 2010 Mar 23. PMID 20203010, Free PMC Article

See all (24) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that LptD is folded around LptE, and both components interact with the two essential beta-barrel assembly machine (Bam) components, BamA and BamD.
    Title: Characterization of a stalled complex on the β-barrel assembly machine.
  2. LptE binds to and alters the physical state of LPS to catalyze its assembly at the cell surface.
    Title: LptE binds to and alters the physical state of LPS to catalyze its assembly at the cell surface.
  3. findings suggest lptE14 causes poor plugging of the LptD beta barrel and that a reduction of ineffectively plugged LptD-LptE14 complexes in the outer membrane decreases permeability to erythromycin; lptE14 supports a proposed plug-and-barrel LptE-LptD arrangement
    Title: Dominant negative lptE mutation that supports a role for LptE as a plug in the LptD barrel.
  4. Data show that oxidation of LptD is not required for the formation of the LptD/E complex but it is essential for function.
    Title: Nonconsecutive disulfide bond formation in an essential integral outer membrane protein.
  5. LptD contains a soluble N-terminal domain and a C-terminal transmembrane domain. LptE stabilizes LptD by interacting with the C-terminal domain of LptD. LptE binds LPS specifically and may serve as a substrate recognition site at the outer membrane.
    Title: Characterization of the two-protein complex in Escherichia coli responsible for lipopolysaccharide assembly at the outer membrane.
  6. Results show that RlpB is essential for cell viability and that the Imp/RlpB complex is responsible for lipopolysaccharides reaching the outer surface of the outer membrane.
    Title: Identification of a protein complex that assembles lipopolysaccharide in the outer membrane of Escherichia coli.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables lipopolysaccharide binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables lipopolysaccharide binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in Gram-negative-bacterium-type cell outer membrane assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in Gram-negative-bacterium-type cell outer membrane assembly IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within Gram-negative-bacterium-type cell outer membrane assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in Gram-negative-bacterium-type cell outer membrane assembly NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in lipopolysaccharide transport IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within lipopolysaccharide transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in lipopolysaccharide transport NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cell outer membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cell outer membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cell outer membrane IEA
Inferred from Electronic Annotation
more info
  
located_in cell outer membrane NAS
Non-traceable Author Statement
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in outer membrane IEA
Inferred from Electronic Annotation
more info
  
part_of transporter complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
lipopolysaccharide assembly protein LptE
NP_415174.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    671605..672186 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415174.1 lipopolysaccharide assembly protein LptE [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415174.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9ADK9, A0A6M9WT04, A0A7D7PT64, A0A8S7EG23
    Conserved Domains (1) summary
    PRK10796
    Location:1 → 189
    PRK10796; LPS-assembly lipoprotein RlpB; Provisional
Nucleotide Protein
Heading Accession and Version

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