U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

otsA trehalose-6-phosphate synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946405, updated on 11-Apr-2024

Summary

Go to the top of the page Help
Gene symbol
otsA
Gene description
trehalose-6-phosphate synthase
Primary source
ASAP:ABE-0006318
Locus tag
b1896
See related
ECOCYC:EG11751
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1895
Summary
Under conditions of high osmolarity, Escherichia coli synthesizes high concentrations of internal trehalose. [More information is available at EcoCyc: EG11751].
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Sequence:
NC_000913.3 (1980188..1981612, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene IS1 family protein InsB Neighboring gene IS1 family protein InsA Neighboring gene universal stress protein C Neighboring gene trehalose-6-phosphate phosphatase Neighboring gene arabinose ABC transporter membrane subunit Neighboring gene arabinose ABC transporter ATP binding subunit

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Trehalose protects Escherichia coli against carbon stress manifested by protein acetylation and aggregation. Moruno Algara M, et al. Mol Microbiol, 2019 Sep. PMID 31162854
  2. Enhanced trehalose production improves growth of Escherichia coli under osmotic stress. Purvis JE, et al. Appl Environ Microbiol, 2005 Jul. PMID 16000787, Free PMC Article
  3. The donor subsite of trehalose-6-phosphate synthase: binary complexes with UDP-glucose and UDP-2-deoxy-2-fluoro-glucose at 2 A resolution. Gibson RP, et al. J Biol Chem, 2004 Jan 16. PMID 14570926
  4. Insights into trehalose synthesis provided by the structure of the retaining glucosyltransferase OtsA. Gibson RP, et al. Chem Biol, 2002 Dec. PMID 12498887
  5. Characterization of Escherichia coli OtsA, a trehalose-6-phosphate synthase from glycosyltransferase family 20. Gibson RP, et al. Acta Crystallogr D Biol Crystallogr, 2002 Feb. PMID 11807274

See all (24) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Deletion of otsA resulted in lack of trehalose synthesis and leaded to increased protein aggregation during the stationary phase.
    Title: Trehalose protects Escherichia coli against carbon stress manifested by protein acetylation and aggregation.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex.
Submit: New GeneRIF Correction

General gene information

Go to the top of the page Help

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables glycosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables hexosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within DNA damage response IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within response to osmotic stress IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within response to osmotic stress IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in trehalose biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within trehalose biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within trehalose metabolism in response to cold stress IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within trehalose metabolism in response to cold stress IMP
Inferred from Mutant Phenotype
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
trehalose-6-phosphate synthase
NP_416410.1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1980188..1981612 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416410.1 trehalose-6-phosphate synthase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416410.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5D8MWE0
    Conserved Domains (1) summary
    PRK10117
    Location:1 → 474
    PRK10117; trehalose-6-phosphate synthase; Provisional
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.