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pykA pyruvate kinase 2 [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946527, updated on 4-Apr-2024

Summary

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Gene symbol
pykA
Gene description
pyruvate kinase 2
Primary source
ASAP:ABE-0006182
Locus tag
b1854
See related
ECOCYC:EG10803
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1855
Summary
PykA contributes significantly less than PykF to in vivo pyruvate kinase activity (Ponce, 1995; Al Zaid Siddiquee, 2004). [More information is available at EcoGene: EG10803]. [More information is available at EcoCyc: EG10803].
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Genomic context

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Sequence:
NC_000913.3 (1937649..1939091)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene NADP(+)-dependent glucose-6-phosphate dehydrogenase Neighboring gene DNA-binding transcriptional repressor YebK Neighboring gene Lipid A biosynthesis myristoyltransferase Neighboring gene peptidoglycan endopeptidase MepM

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Reexamination of the Physiological Role of PykA in Escherichia coli Revealed that It Negatively Regulates the Intracellular ATP Levels under Anaerobic Conditions. Zhao C, et al. Appl Environ Microbiol, 2017 Jun 1. PMID 28363967, Free PMC Article
  2. Recombinant pyruvate kinase type I from Escherichia coli: overproduction and revised C-terminus of the polypeptide. Valentini G, et al. Biol Chem, 1997 Jul. PMID 9278153
  3. Purification and molecular properties of the AMP-activated pyruvate kinase from Escherichia coli. Somani BL, et al. Biochim Biophys Acta, 1977 May 12. PMID 193572
  4. Pyruvate kinase-deficient Escherichia coli exhibits increased plasmid copy number and cyclic AMP levels. Cunningham DS, et al. J Bacteriol, 2009 May. PMID 19251844, Free PMC Article
  5. A bacterial selection for the directed evolution of pyruvate aldolases. Griffiths JS, et al. Bioorg Med Chem, 2004 Aug 1. PMID 15246084

See all (37) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Knocking out pykA significantly increased the intracellular ATP level and thus significantly increased the levels of glucose consumption and biomass formation.
    Title: Reexamination of the Physiological Role of PykA in Escherichia coli Revealed that It Negatively Regulates the Intracellular ATP Levels under Anaerobic Conditions.
  2. The elevated cAMP in PB25 suggests that pyruvate kinase may participate in glucose catabolite repression by serving among all of the factors that tighten gene expression.
    Title: Pyruvate kinase-deficient Escherichia coli exhibits increased plasmid copy number and cyclic AMP levels.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: Bacterial pyruvate kinases have a shorter N-terminal domain.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables AMP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables potassium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables pyruvate kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables pyruvate kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyruvate kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in glycolytic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in glycolytic process IEA
Inferred from Electronic Annotation
more info
  
involved_in metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in phosphorylation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of pyruvate kinase complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
pyruvate kinase 2
NP_416368.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1937649..1939091
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416368.1 pyruvate kinase 2 [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416368.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AJF4
    Conserved Domains (1) summary
    PRK05826
    Location:3 → 469
    PRK05826; pyruvate kinase; Provisional
Nucleotide Protein
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