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glpT sn-glycerol 3-phosphate:phosphate antiporter [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946704, updated on 2-May-2024

Summary

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Gene symbol
glpT
Gene description
sn-glycerol 3-phosphate:phosphate antiporter
Primary source
ASAP:ABE-0007401
Locus tag
b2240
See related
ECOCYC:EG10401
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2232
Summary
GlpT exhanges cytoplasmic phosphate for glycerol phosphate. [More information is available at EcoGene: EG10401]. GlpT is the major Escherichia coli uptake system for glycerol-3-phosphate, and this transporter belongs to the Major Facilitator Superfamily (MFS) of transporters . [More information is available at EcoCyc: EG10401].
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Genomic context

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Sequence:
NC_000913.3 (2351016..2352374, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene pseudo Neighboring gene glycerophosphoryl diester phosphodiesterase GlpQ Neighboring gene anaerobic glycerol-3-phosphate dehydrogenase subunit A Neighboring gene anaerobic glycerol-3-phosphate dehydrogenase subunit B Neighboring gene anaerobic glycerol-3-phosphate dehydrogenase subunit C

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structural basis of substrate selectivity in the glycerol-3-phosphate: phosphate antiporter GlpT. Law CJ, et al. Biophys J, 2009 Sep 2. PMID 19720022, Free PMC Article
  2. Helix dynamics in a membrane transport protein: comparative simulations of the glycerol-3-phosphate transporter and its constituent helices. D'Rozario RS, et al. Mol Membr Biol, 2008 Sep. PMID 19037818
  3. Modeling of glycerol-3-phosphate transporter suggests a potential 'tilt' mechanism involved in its function. Tsigelny IF, et al. J Bioinform Comput Biol, 2008 Oct. PMID 18942157, Free PMC Article
  4. Salt-bridge dynamics control substrate-induced conformational change in the membrane transporter GlpT. Law CJ, et al. J Mol Biol, 2008 May 9. PMID 18395745, Free PMC Article
  5. Kinetic evidence is consistent with the rocker-switch mechanism of membrane transport by GlpT. Law CJ, et al. Biochemistry, 2007 Oct 30. PMID 17915951, Free PMC Article

See all (74) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Elevated expression of GlpT and UhpT via FNR activation contributes to increased fosfomycin susceptibility in Escherichia coli under anaerobic conditions.
    Title: Elevated Expression of GlpT and UhpT via FNR Activation Contributes to Increased Fosfomycin Susceptibility in Escherichia coli under Anaerobic Conditions.
  2. Data indicate that mutating noninteracting residues to alanine have no effect on the pulling force of C-terminal transmembrane helices (TMHs) in membrane transport proteins CaiT, BtuC, NhaA, EmrD and GlpT.
    Title: Cotranslational folding of membrane proteins probed by arrest-peptide-mediated force measurements.
  3. studies suggest that only a few amino acid residues that line the GlpT transporter lumen act as specificity determinants
    Title: Structural basis of substrate selectivity in the glycerol-3-phosphate: phosphate antiporter GlpT.
  4. simulations highlight the cavity-lining helices as being significantly flexible, suggesting that the transport mechanism may involve intra-helix motions in addition to pseudo-rigid body motions of the N- and C-terminal domains relative to one another
    Title: Helix dynamics in a membrane transport protein: comparative simulations of the glycerol-3-phosphate transporter and its constituent helices.
  5. Here we report the 3.3 A resolution structure of GlpT, the glycerol-3-phosphate transporter from the E. coli inner membrane, in the absence of a substrate and propose that GlpT operates via a single binding-site, alternating-access mechanism.
    Title: Crystal structure and mechanism of GlpT, the glycerol-3-phosphate transporter from E. coli.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables glucose 6-phosphate:phosphate antiporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glycerol-3-phosphate transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables glycerol-phosphate:phosphate antiporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables organophosphate:phosphate antiporter activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in glucose-6-phosphate transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in glycerol metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glycerol transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within glycerol-3-phosphate transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in glycerol-3-phosphate transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in phosphate ion transmembrane transport IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 

General protein information

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Preferred Names
sn-glycerol 3-phosphate:phosphate antiporter

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2351016..2352374 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416743.1 sn-glycerol 3-phosphate:phosphate antiporter [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416743.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7EAF3
    Conserved Domains (1) summary
    PRK11273
    Location:1 → 452
    glpT; glycerol-3-phosphate transporter
Nucleotide Protein
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