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nuoB NADH:quinone oxidoreductase subunit B [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946738, updated on 4-Feb-2024

Summary

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Gene symbol
nuoB
Gene description
NADH:quinone oxidoreductase subunit B
Primary source
ASAP:ABE-0007551
Locus tag
b2287
See related
ECOCYC:EG12083
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2281
Summary
NuoB has iron-sulfur cluster N2. [More information is available at EcoGene: EG12083]. NuoB is part of the connecting fragment of NADH dehydrogenase I . [More information is available at EcoCyc: EG12083].
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Genomic context

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Sequence:
NC_000913.3 (2403951..2404613, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene NADH:quinone oxidoreductase subunit F Neighboring gene NADH:quinone oxidoreductase subunit E Neighboring gene NADH:quinone oxidoreductase subunit CD Neighboring gene NADH:quinone oxidoreductase subunit A Neighboring gene DNA-binding transcriptional dual regulator LrhA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A possible role for iron-sulfur cluster N2 in proton translocation by the NADH: ubiquinone oxidoreductase (complex I). Flemming D, et al. J Mol Microbiol Biotechnol, 2005. PMID 16645316
  2. Involvement of tyrosines 114 and 139 of subunit NuoB in the proton pathway around cluster N2 in Escherichia coli NADH:ubiquinone oxidoreductase. Flemming D, et al. J Biol Chem, 2003 Jan 31. PMID 12446673
  3. Catalytic importance of acidic amino acids on subunit NuoB of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I). Flemming D, et al. J Biol Chem, 2006 Aug 25. PMID 16807239
  4. Spin labeling of the Escherichia coli NADH ubiquinone oxidoreductase (complex I). Pohl T, et al. Biochim Biophys Acta, 2010 Dec. PMID 20959113
  5. Chemical and NADH-induced, ROS-dependent, cross-linking between subunits of complex I from Escherichia coli and Thermus thermophilus. Berrisford JM, et al. Biochemistry, 2008 Sep 30. PMID 18771280

See all (65) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Study determined the distance between a MTSL labeled complex I variant and the bound quinone by continuous-wave (cw) EPR allowing an inference on the location of the quinone binding site.
    Title: Spin labeling of the Escherichia coli NADH ubiquinone oxidoreductase (complex I).
  2. N-terminus verified by Edman degradation on complete protein
    Title: Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables 4 iron, 4 sulfur cluster binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables NADH dehydrogenase (quinone) activity IEA
Inferred from Electronic Annotation
more info
  
contributes_to NADH dehydrogenase (ubiquinone) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables NADH dehydrogenase (ubiquinone) activity IEA
Inferred from Electronic Annotation
more info
  
contributes_to NADH dehydrogenase (ubiquinone) activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
contributes_to NADH dehydrogenase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables iron ion binding IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables quinone binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in aerobic respiration IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within aerobic respiration IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in electron transport coupled proton transport IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within electron transport coupled proton transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in respiratory electron transport chain IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of NADH dehydrogenase complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane HDA PubMed 
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
part_of plasma membrane respiratory chain complex I IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of plasma membrane respiratory chain complex I IDA
Inferred from Direct Assay
more info
 PubMed 
part_of plasma membrane respiratory chain complex I IMP
Inferred from Mutant Phenotype
more info
 PubMed 
part_of respiratory chain complex I NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
NADH:quinone oxidoreductase subunit B
NP_416790.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2403951..2404613 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416790.1 NADH:quinone oxidoreductase subunit B [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416790.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A7H4YJW6
    Conserved Domains (1) summary
    COG0377
    Location:17 → 210
    NuoB; NADH:ubiquinone oxidoreductase 20 kD subunit (chhain B) or related Fe-S oxidoreductase [Energy production and conversion]
Nucleotide Protein
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