ptsH phosphocarrier protein HPr [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: ptsH
Gene description: phosphocarrier protein HPr
Primary source: ASAP:ABE-0007962
Locus tag: b2415
See related: ECOCYC:EG10788
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ctr; ECK2410; hpr
Summary: HPr (heat stable, histidyl phosphorylatable protein) is the second of two sugar-non-specific protein constituents of the PTS . [More information is available at EcoCyc: EG10788].
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Genomic context

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Sequence:: NC_000913.3 (2533764..2534021)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Glucose preference over another phosphoenolpyruvate:sugar phosphotransferase system (PTS) sugar, mannitol, is absolutely dependent on the general PTS component HPr, but not on EIIAGlc, in E. coli. Dephosphorylated HPr accumulates during the transport of glucose and interacts with the mannitol operon regulator, MtlR, to augment its repressor activity.
Title: The general PTS component HPr determines the preference for glucose over mannitol.
HPr is either phosphorylated on a histidyl residue (HPr-P) or non-phosphorylated (HPr). Data suggest HPr (the non-phosphorylated form) participates in allosteric regulation of the activities of pyruvate kinase (PykF, but not PykA), phosphofructokinase (PfkB, but not PfkA), glucosamine-6-phosphate deaminase (NagB), and adenylate kinase (Adk).
Title: The phosphocarrier protein HPr of the bacterial phosphotransferase system globally regulates energy metabolism by directly interacting with multiple enzymes in Escherichia coli.
Results suggest that the phosphoenolpyruvate hexose phosphotransferases PTS network serves as a global sensor of the sugar influx and also monitors the levels of several non-PTS compounds.
Title: Sugar Influx Sensing by the Phosphotransferase System of Escherichia coli.
HPr antagonizes the anti-sigma70 activity of Rsd in Escherichia coli.
Title: HPr antagonizes the anti-σ70 activity of Rsd in Escherichia coli.
Data show the active site histidine of histidine phosphocarrier protein HPr, His-15, is buried within the active site cleft of IIA(Chb) formed at the interface of two adjacent subunits of the IIA(Chb) trimer.
Title: Solution structure of the IIAChitobiose-HPr complex of the N,N'-diacetylchitobiose branch of the Escherichia coli phosphotransferase system.
N-terminus verified by Edman degradation on complete protein
Title: Acid- and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables antisigma factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables enzyme activator activity	IDA Inferred from Direct Assay more info	PubMed
enables enzyme inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables enzyme regulator activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphotransferase activity, nitrogenous group as acceptor	TAS Traceable Author Statement more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in organic substance transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within phosphoenolpyruvate-dependent sugar phosphotransferase system	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphoenolpyruvate-dependent sugar phosphotransferase system	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of glycogen catabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of carbon utilization	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: phosphocarrier protein HPr

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

2533764..2534021

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_416910.1 phosphocarrier protein HPr [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_416910.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A8S0FQV4

Conserved Domains (1) summary

PRK10850
Location:1 → 85

PRK10850; phosphocarrier protein Hpr