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cysM cysteine synthase B [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946888, updated on 4-Apr-2024

Summary

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Gene symbol
cysM
Gene description
cysteine synthase B
Primary source
ASAP:ABE-0007982
Locus tag
b2421
See related
ECOCYC:EG10193
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2416
Summary
Cysteine synthase B is one of two isozymes in Escherichia coli that catalyze the formation of L-cysteine from O-acetyl-L-serine and sulfide . [More information is available at EcoCyc: EG10193].
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Genomic context

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Sequence:
NC_000913.3 (2538672..2539583, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF5329 domain-containing protein YfeK Neighboring gene PF05406 family protein YfeS Neighboring gene sulfate/thiosulfate ABC transporter ATP binding subunit Neighboring gene sulfate/thiosulfate ABC transporter inner membrane subunit CysW Neighboring gene sulfate/thiosulfate ABC transporter inner membrane subunit CysU Neighboring gene thiosulfate/sulfate ABC transporter periplasmic binding protein CysP

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Cysteine homeostasis under inhibition of protein synthesis in Escherichia coli cells. Smirnova GV, et al. Amino Acids, 2019 Nov. PMID 31617110
  2. High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli. Zocher G, et al. FEBS J, 2007 Oct. PMID 17894825
  3. Cloning, overexpression, purification, and characterization of O-acetylserine sulfhydrylase-B from Escherichia coli. Zhao C, et al. Protein Expr Purif, 2006 Jun. PMID 16546401
  4. Structure of the O-acetylserine sulfhydrylase isoenzyme CysM from Escherichia coli. Claus MT, et al. Biochemistry, 2005 Jun 21. PMID 15952768
  5. Semisynthetic production of unnatural L-alpha-amino acids by metabolic engineering of the cysteine-biosynthetic pathway. Maier TH. Nat Biotechnol, 2003 Apr. PMID 12640465

See all (25) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on complete protein
    Title: Studies on the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase in escherichia coli crude extract. Isolation of O-acetylserine sulfhydrylases A and B and beta-cystathionase based on their ability to mobilize sulfur from cysteine and to participate in Fe-S cluster synthesis.
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Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables L-cysteine desulfhydrase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables cysteine synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables cysteine synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cysteine synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxal phosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity, transferring alkyl or aryl (other than methyl) groups IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within L-cysteine catabolic process to pyruvate IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in cysteine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in cysteine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cysteine biosynthetic process from serine IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cysteine biosynthetic process from serine IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cysteine biosynthetic process via S-sulfo-L-cysteine IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within iron-sulfur cluster assembly IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
cysteine synthase B
NP_416916.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2538672..2539583 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416916.1 cysteine synthase B [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416916.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A827XX18
    Conserved Domains (1) summary
    PRK11761
    Location:1 → 294
    cysM; cysteine synthase CysM
Nucleotide Protein
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