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amiA N-acetylmuramoyl-L-alanine amidase A [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946916, updated on 4-Feb-2024

Summary

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Gene symbol
amiA
Gene description
N-acetylmuramoyl-L-alanine amidase A
Primary source
ASAP:ABE-0008032
Locus tag
b2435
See related
ECOCYC:EG11823
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2430; yfeE
Summary
Verified Tat substrate: [More information is available at EcoGene: EG11823]. Escherichia coli contains three N-acetylmuramyl-L-alanine amidases named AmiA, B, and C involved in cell division by splitting the murein septum. [More information is available at EcoCyc: EG11823].
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Genomic context

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Sequence:
NC_000913.3 (2552352..2553221)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF1131 domain-containing lipoprotein YfeY Neighboring gene putative inner membrane protein Neighboring gene putative acetyltransferase YpeA Neighboring gene coproporphyrinogen III oxidase Neighboring gene putative DNA-binding transcriptional regulator EutR

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Studying a cell division amidase using defined peptidoglycan substrates. Lupoli TJ, et al. J Am Chem Soc, 2009 Dec 30. PMID 19957935, Free PMC Article
  2. Mutation of the N-acetylmuramyl-L-alanine amidase gene of Escherichia coli K-12. Tomioka S, et al. J Bacteriol, 1983 Oct. PMID 6137479, Free PMC Article
  3. Nature of the interactions involved in the lipid-protein complexes of the Escherichia coli N-acetylmuramoyl-L-alanine amidase. Vanderwinkel E, et al. Biochim Biophys Acta, 1987 Jun 17. PMID 2885030
  4. Modulation of Escherichia coli N-acetylmuramoyl-L-alanine amidase activity by phosphatidylglycerol. Vanderwinkel E, et al. Biochim Biophys Acta, 1985 Jan 28. PMID 2857097
  5. Systematic characterization of curved DNA segments randomly cloned from Escherichia coli and their functional significance. Tanaka K, et al. Mol Gen Genet, 1991 May. PMID 1903834

See all (27) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. AmiA is a zinc metalloprotease that requires at least a tetrasaccharide glycopeptide substrate for cleavage.
    Title: Studying a cell division amidase using defined peptidoglycan substrates.
  2. Periplasmic amidases AmiC and AmiA, secreted by the twin arginine transport (Tat) pathway, are localized to the cytoplasm in Escherichia coli YqjA and YghB double mutant cells.
    Title: Inefficient Tat-dependent export of periplasmic amidases in an Escherichia coli strain with mutations in two DedA family genes.
Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables N-acetylmuramoyl-L-alanine amidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables N-acetylmuramoyl-L-alanine amidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables N-acetylmuramoyl-L-alanine amidase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in cell wall organization IEA
Inferred from Electronic Annotation
more info
  
involved_in cytokinesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in peptidoglycan catabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in outer membrane-bounded periplasmic space IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in outer membrane-bounded periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in outer membrane-bounded periplasmic space ISM
Inferred from Sequence Model
more info
 PubMed 
located_in periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in periplasmic space IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
N-acetylmuramoyl-L-alanine amidase A
NP_416930.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2552352..2553221
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416930.1 N-acetylmuramoyl-L-alanine amidase A [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416930.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8T5Y666
    Conserved Domains (1) summary
    PRK10319
    Location:1 → 287
    PRK10319; N-acetylmuramoyl-L-alanine amidase AmiA
Nucleotide Protein
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