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cysS cysteine--tRNA ligase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946969, updated on 2-May-2024

Summary

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Gene symbol
cysS
Gene description
cysteine--tRNA ligase
Primary source
ASAP:ABE-0001810
Locus tag
b0526
See related
ECOCYC:EG10196
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0519; syc
Summary
Cysteinyl-tRNA synthetase (CysRS) is a member of the family of aminoacyl-tRNA synthetases, which interpret the genetic code by covalently linking amino acids to their specific tRNA molecules. [More information is available at EcoCyc: EG10196].
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Genomic context

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Sequence:
NC_000913.3 (554611..555996)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene UDP-2,3-diacylglucosamine diphosphatase Neighboring gene peptidyl-prolyl cis-trans isomerase B Neighboring gene PF04307 family inner membrane protein YbcI Neighboring gene putative RNA-binding protein YbcJ

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Pyrrolo-C as a molecular probe for monitoring conformations of the tRNA 3' end. Zhang CM, et al. RNA, 2008 Oct. PMID 18755841, Free PMC Article
  2. Domain-domain communication for tRNA aminoacylation: the importance of covalent connectivity. Zhang CM, et al. Biochemistry, 2005 May 17. PMID 15882062
  3. Breaking the stereo barrier of amino acid attachment to tRNA by a single nucleotide. Shitivelband S, et al. J Mol Biol, 2005 May 6. PMID 15826650
  4. Shape-selective RNA recognition by cysteinyl-tRNA synthetase. Hauenstein S, et al. Nat Struct Mol Biol, 2004 Nov. PMID 15489861
  5. Amino acid discrimination by a highly differentiated metal center of an aminoacyl-tRNA synthetase. Zhang CM, et al. Biochemistry, 2003 Sep 23. PMID 12974627

See all (33) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on complete protein
    Title: Cysteinyl-tRNA synthetase: determination of the last E. coli aminoacyl-tRNA synthetase primary structure.
  2. cysteinyl-tRNA synthetase recognizes RNA in a shape specific manner
    Title: Shape-selective RNA recognition by cysteinyl-tRNA synthetase.
  3. Long-range covalent continuity between the active site domain at the N-terminus of cysteine tRNA synthetase and the cysteinyl-tRNA-binding anticodon domain at the enzyme C-terminus is required for efficient tRNA aminoacylaton.
    Title: Domain-domain communication for tRNA aminoacylation: the importance of covalent connectivity.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables aminoacyl-tRNA ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables aminoacyl-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables cysteine-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables cysteine-tRNA ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cysteine-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in cysteinyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within cysteinyl-tRNA aminoacylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cysteinyl-tRNA aminoacylation IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA aminoacylation for protein translation IEA
Inferred from Electronic Annotation
more info
  
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
cysteine--tRNA ligase
NP_415059.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    554611..555996
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415059.1 cysteine--tRNA ligase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415059.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A2W6Q295
    Conserved Domains (1) summary
    PRK00260
    Location:1 → 461
    cysS; cysteinyl-tRNA synthetase; Validated
Nucleotide Protein
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Gene LinkOut

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