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ispG (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin) [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946991, updated on 4-Feb-2024

Summary

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Gene symbol
ispG
Gene description
(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin)
Primary source
ASAP:ABE-0008281
Locus tag
b2515
See related
ECOCYC:EG10370
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2511; gcpE
Summary
The IspG substrate is 2C-methyl-d-erythritol 2,4-cyclodiphosphate. [More information is available at EcoGene: EG10370]. 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (IspG) catalyzes the sixth step in the methylerythritol phosphate pathway. [More information is available at EcoCyc: EG10370].
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Genomic context

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Sequence:
NC_000913.3 (2640686..2641804, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene histidine--tRNA ligase Neighboring gene ncRNA Neighboring gene cytoskeleton protein RodZ Neighboring gene 23S rRNA m(2)A2503 methyltransferase/tRNA m(2)A37 methyltransferase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. IspG converts an epoxide substrate analogue to (E)-4-hydroxy-3-methylbut-2-enyl diphosphate: implications for IspG catalysis in isoprenoid biosynthesis. Nyland RL 2nd, et al. J Am Chem Soc, 2009 Dec 16. PMID 19919056, Free PMC Article
  2. IspG enzyme activity in the deoxyxylulose phosphate pathway: roles of the iron-sulfur cluster. Xiao Y, et al. Biochemistry, 2009 Nov 10. PMID 19821611
  3. Biosynthesis of isoprenoids. purification and properties of IspG protein from Escherichia coli. Zepeck F, et al. J Org Chem, 2005 Nov 11. PMID 16268586
  4. Isoprenoid biosynthesis through the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE) is a [4Fe-4S] protein. Seemann M, et al. Angew Chem Int Ed Engl, 2002 Nov 15. PMID 12434382
  5. Cutting edge: human gamma delta T cells are activated by intermediates of the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis. Altincicek B, et al. J Immunol, 2001 Mar 15. PMID 11238603

See all (30) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. work for the first time identified HMBPP to be a cytotoxic intermediate in MEP pathway and demonstrated that balanced activation of IspG and IspH could eliminate accumulation of HMBPP and MECPP and improve isoprenoids production.
    Title: Balanced activation of IspG and IspH to eliminate MEP intermediate accumulation and improve isoprenoids production in Escherichia coli.
  2. analysis of the organometallic mechanism of action and inhibition of the 4Fe-4S isoprenoid biosynthesis protein GcpE (IspG)
    Title: Organometallic mechanism of action and inhibition of the 4Fe-4S isoprenoid biosynthesis protein GcpE (IspG).
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 4 iron, 4 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables iron ion binding IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in isoprenoid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in terpenoid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin)
NP_417010.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2640686..2641804 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417010.1 (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin) [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417010.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7YIH4
    Conserved Domains (1) summary
    PRK00366
    Location:1 → 359
    ispG; flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase
Nucleotide Protein
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