iscU scaffold protein for iron-sulfur cluster assembly [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: iscU
Gene description: scaffold protein for iron-sulfur cluster assembly
Primary source: ASAP:ABE-0008321
Locus tag: b2529
See related: ECOCYC:G7324
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK2526; nifU; yfhN
Summary: IscU fails to assemble Fe-S clusters in the presence of sodium citrate due to restricted free iron availability, however Fe-S cluster formation is restored, with sodium citrate present, in the presence of IscA. [More information is available at EcoGene: EG13395]. The iscSUA operon plays a role in assembly (or maintenance) of iron-sulfur clusters . [More information is available at EcoCyc: G7324].
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Genomic context

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Sequence:: NC_000913.3 (2659903..2660289, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Evidence for dynamic in vivo interconversion of the conformational states of IscU during iron-sulfur cluster biosynthesis.
Title: Evidence for dynamic in vivo interconversion of the conformational states of IscU during iron-sulfur cluster biosynthesis.
We have demonstrated that a single mutation in the scaffold protein IscU rendered E. coli viability strictly dependent upon a functional CyaY.
Title: Turning Escherichia coli into a Frataxin-Dependent Organism.
Data indicate that the single point mutation IscU_D39A affects the 2Fe-2S coordination geometry.
Title: Anatomy of an iron-sulfur cluster scaffold protein: Understanding the determinants of [2Fe-2S] cluster stability on IscU.
Data indicate that IscU exists as two interconverting species: one (S) is largely structured with all four peptidyl-prolyl bonds trans; the other (D) contains an ordered domain that stabilizes two cis peptidyl-prolyl peptide bonds.
Title: Metamorphic protein IscU alternates conformations in the course of its role as the scaffold protein for iron-sulfur cluster biosynthesis and delivery.
The iron-sulfur cluster assembly in E. coli IscU is an IscS mediated biosynthetic process.
Title: Biochemical characterization of iron-sulfur cluster assembly in the scaffold IscU of Escherichia coli.
study determined the crystal structures of IscS-IscU and IscS-TusA complexes providing the first insight into their different modes of binding and the mechanism of sulfur transfer
Title: Structural basis for Fe-S cluster assembly and tRNA thiolation mediated by IscS protein-protein interactions.
Thioredoxin reductase system mediates iron binding in IscA and iron delivery for the iron-sulfur cluster assembly in IscU
Title: Thioredoxin reductase system mediates iron binding in IscA and iron delivery for the iron-sulfur cluster assembly in IscU.
Escherichia coli CYaY is an iron donor for the assembly of [2Fe-2S] clusters in the scaffold IscU
Title: Iron-sulfur cluster biosynthesis: characterization of Escherichia coli CYaY as an iron donor for the assembly of [2Fe-2S] clusters in the scaffold IscU.
both iron-sulfur cluster assembly protein IscU and co-chaperone HscB interact with the low peptide affinity T-state conformation of HscA ATPase and both modestly accelerate the rate-determining steps in the HscA reaction cycle
Title: Regulation of the HscA ATPase reaction cycle by the co-chaperone HscB and the iron-sulfur cluster assembly protein IscU.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables 2 iron, 2 sulfur cluster binding	IBA Inferred from Biological aspect of Ancestor more info
enables 2 iron, 2 sulfur cluster binding	IDA Inferred from Direct Assay more info	PubMed
enables 4 iron, 4 sulfur cluster binding	IDA Inferred from Direct Assay more info	PubMed
enables copper ion binding	IDA Inferred from Direct Assay more info	PubMed
enables ferrous iron binding	IBA Inferred from Biological aspect of Ancestor more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables iron-sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in L-cysteine catabolic process	NAS Non-traceable Author Statement more info	PubMed
involved_in intracellular iron ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in iron-sulfur cluster assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in iron-sulfur cluster assembly	IEA Inferred from Electronic Annotation more info
involved_in iron-sulfur cluster assembly	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of IscS-IscU complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: scaffold protein for iron-sulfur cluster assembly

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

2659903..2660289 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417024.1 scaffold protein for iron-sulfur cluster assembly [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417024.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A5B9AR62

Conserved Domains (1) summary

PRK11325
Location:1 → 127

PRK11325; scaffold protein; Provisional