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iscS cysteine desulfurase IscS [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947004, updated on 4-Feb-2024

Summary

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Gene symbol
iscS
Gene description
cysteine desulfurase IscS
Primary source
ASAP:ABE-0008324
Locus tag
b2530
See related
ECOCYC:G7325
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2527; nuvC; yfhO; yzzO
Summary
IscS desulfurase from cysteine via a Cys328 persulfide to binding partners as a transpersulfidase with ThiI Cys456 for tRNA s(4)U8 modification and with SirA(TusA) Cys19 to thiolate tRNA U34. [More information is available at EcoGene: EG12677]. Cysteine desulfurase (IcsC) catalyzes the transfer of sulfur and selenium from cysteine and selenocysteine to a range of recipients. [More information is available at EcoCyc: G7325].
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Genomic context

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Sequence:
NC_000913.3 (2660317..2661531, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene iron-sulfur cluster insertion protein IscA Neighboring gene scaffold protein for iron-sulfur cluster assembly Neighboring gene DNA-binding transcriptional dual regulator IscR Neighboring gene tRNA Cm32/Um32 methyltransferase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Regulation of Escherichia coli IscS desulfurase activity by ferrous iron and cysteine. Wu G, et al. Biochem Biophys Res Commun, 2008 Sep 19. PMID 18639525
  2. The cysteine-desulfurase IscS promotes the production of the rhodanese RhdA in the persulfurated form. Forlani F, et al. FEBS Lett, 2005 Dec 19. PMID 16310786
  3. Bacterial cysteine desulfurases: their function and mechanisms. Mihara H, et al. Appl Microbiol Biotechnol, 2002 Oct. PMID 12382038
  4. Preliminary crystallographic analysis of the cysteine desulfurase IscS from Escherichia coli. Urbina HD, et al. Acta Crystallogr D Biol Crystallogr, 2002 Jul. PMID 12077450
  5. Repair of nitric oxide-modified ferredoxin [2Fe-2S] cluster by cysteine desulfurase (IscS). Yang W, et al. J Biol Chem, 2002 Apr 12. PMID 11825893

See all (79) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. human NFS1 was almost fully able to complement the role of IscS in Moco biosynthesis when its specific interaction partner protein MOCS3 from humans was also present.
    Title: Functional Complementation Studies Reveal Different Interaction Partners of Escherichia coli IscS and Human NFS1.
  2. TusA (YhhP) and IscS are required for molybdenum cofactor-dependent base-analog detoxification.
    Title: TusA (YhhP) and IscS are required for molybdenum cofactor-dependent base-analog detoxification.
  3. study determined the crystal structures of IscS-IscU and IscS-TusA complexes providing the first insight into their different modes of binding and the mechanism of sulfur transfer
    Title: Structural basis for Fe-S cluster assembly and tRNA thiolation mediated by IscS protein-protein interactions.
  4. CyaY participates in iron-sulfur (Fe-S) cluster assembly as an iron-dependent inhibitor of cluster formation, through binding to the desulfurase IscS.
    Title: Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS.
  5. The desulfurase activity of IscS is gradually inhibited as the amount of iron and sulfide bound to IscS increases.
    Title: Regulation of Escherichia coli IscS desulfurase activity by ferrous iron and cysteine.
  6. These results provide a clue to the possible role of iscS in pyrimidine metabolism by gene regulation.
    Title: The iscS gene deficiency affects the expression of pyrimidine metabolism genes.
  7. N-terminus verified by Edman degradation on complete protein
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 2 iron, 2 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables cysteine desulfurase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cysteine desulfurase activity IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables pyridoxal phosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxal phosphate binding IEA
Inferred from Electronic Annotation
more info
  
enables selenocysteine lyase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables sulfur carrier activity EXP
Inferred from Experiment
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in L-cysteine catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in L-cysteine catabolic process NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in [2Fe-2S] cluster assembly IEA
Inferred from Electronic Annotation
more info
  
involved_in detection of UV NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in iron-sulfur cluster assembly IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within iron-sulfur cluster assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in iron-sulfur cluster assembly NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within oxazole or thiazole biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in selenocysteine catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in sulfur compound transport IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within tRNA 4-thiouridine biosynthesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA wobble position uridine thiolation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in thiamine biosynthetic process NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
part_of IscS-IscU complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of IscS-TusA complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of L-cysteine desulfurase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of sulfurtransferase complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
cysteine desulfurase IscS
YP_026169.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2660317..2661531 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. YP_026169.1 cysteine desulfurase IscS [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for YP_026169.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7EPN0, A0A8S7QG68
    Conserved Domains (1) summary
    PRK14012
    Location:1 → 404
    PRK14012; IscS subfamily cysteine desulfurase
Nucleotide Protein
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Other Literature Sources
Molecular Biology Databases
Research Materials