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hmp nitric oxide dioxygenase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947018, updated on 4-Apr-2024

Summary

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Gene symbol
hmp
Gene description
nitric oxide dioxygenase
Primary source
ASAP:ABE-0008396
Locus tag
b2552
See related
ECOCYC:EG10456
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2549; fsrB; hmpA
Summary
Upregulated by GSNO in defined medium (aerobic) as part of the NsrR regulon. hmp mutants are defective in macrophage survival and swarming motility (Stevanin, 2007). [More information is available at EcoGene: EG10456].
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Genomic context

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Sequence:
NC_000913.3 (2685835..2687025)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative DNA-binding transcriptional regulator, NAGC-like Neighboring gene serine hydroxymethyltransferase Neighboring gene nitrogen regulatory protein PII-1 Neighboring gene DNA-binding transcriptional activator GlrR Neighboring gene outer membrane lipoprotein QseG

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Role of flavohemoglobin in combating nitrosative stress in uropathogenic Escherichia coli--implications for urinary tract infection. Svensson L, et al. Microb Pathog, 2010 Sep. PMID 20399845
  2. Flavohemoglobin: structure and reactivity. Bonamore A, et al. IUBMB Life, 2008 Jan. PMID 18379989
  3. Thermodynamic and kinetic characterization of apoHmpH, a fast-folding bacterial globin. Eun YJ, et al. J Mol Biol, 2008 Feb 22. PMID 18187151
  4. The hmp gene encoding the NO-inducible flavohaemoglobin in Escherichia coli confers a protective advantage in resisting killing within macrophages, but not in vitro: links with swarming motility. Stevanin TM, et al. Gene, 2007 Aug 15. PMID 17611046
  5. Escherichia coli Hmp, an "oxygen-binding flavohaemoprotein", produces superoxide anion and self-destructs. Wu G, et al. Arch Microbiol, 2004 Oct. PMID 15340787

See all (56) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. comparison of expression of genes involved in folate metabolism and the hmp gene in strains with intact csgD gene and with deletion in csgD; expression of genes for methylene tetrahydrofolate synthesis and formyltetrahydrofolate recycling was higher in cells with CsgD than those without; expression of hmp was lower in the presence of CsgD
    Title: The influence of CsgD on the expression of genes of folate metabolism and hmp in Escherichia coli K-12.
  2. This study demonstrates, for the first time, that Hmp contributes to the protection of uropathogenic Escherichia coli against nitric oxide-mediated toxicity in vitro.
    Title: Role of flavohemoglobin in combating nitrosative stress in uropathogenic Escherichia coli--implications for urinary tract infection.
  3. HMP catalyzes oxidation of NO to NO(3)(-) (NO-dioxygenase activity) in vivo & in vitro in presence of O(2) & NADH. It also catalyzes reduction of several alkylhydroperoxide substrates into their corresponding alcohols using NADH as electron donor[review]
    Title: Flavohemoglobin: structure and reactivity.
  4. N-terminus verified by Edman degradation on complete protein
    Title: Nitric oxide dioxygenase: an enzymic function for flavohemoglobin.
  5. The lack of swarming motility of the hmp mutant and its aflagellate state suggest that Hmp synthesis is a metabolic burden in the absence of NO-related stresses.
    Title: The hmp gene encoding the NO-inducible flavohaemoglobin in Escherichia coli confers a protective advantage in resisting killing within macrophages, but not in vitro: links with swarming motility.
  6. produces superoxide; during prolonged oxidation of NADH, the spectral signals of Hmp decayed and iron was released in a process prevented by superoxide dismutase or catalase
    Title: Escherichia coli Hmp, an "oxygen-binding flavohaemoprotein", produces superoxide anion and self-destructs.
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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables FAD binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables FAD binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables FAD binding IEA
Inferred from Electronic Annotation
more info
  
enables fatty acid binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables heme binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables heme binding IEA
Inferred from Electronic Annotation
more info
  
enables hydroperoxide reductase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nitric oxide dioxygenase NAD(P)H activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables nitric oxide dioxygenase NAD(P)H activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables nitric oxide dioxygenase NAD(P)H activity IEA
Inferred from Electronic Annotation
more info
  
enables nitric oxide dioxygenase NAD(P)H activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables oxygen binding IEA
Inferred from Electronic Annotation
more info
  
enables oxygen carrier activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in cellular response to nitrosative stress IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in nitric oxide catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in oxygen transport IEA
Inferred from Electronic Annotation
more info
  
involved_in response to nitrosative stress IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to nitrosative stress IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within response to nitrosative stress IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to toxic substance IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in intracellular anatomical structure IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
nitric oxide dioxygenase
NP_417047.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2685835..2687025
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417047.1 nitric oxide dioxygenase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417047.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6N8NG14
    Conserved Domains (1) summary
    PRK13289
    Location:1 → 396
    PRK13289; NO-inducible flavohemoprotein
Nucleotide Protein
Heading Accession and Version

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