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dgt dGTP triphosphohydrolase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947177, updated on 2-May-2024

Summary

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Gene symbol
dgt
Gene description
dGTP triphosphohydrolase
Primary source
ASAP:ABE-0000548
Locus tag
b0160
See related
ECOCYC:EG10225
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0159; optA
Summary
Deoxyguanosinetriphosphate triphosphohydrolase (dGTPase) is a unique nucleoside triphosphatase in that it hydrolyzes dGTP to deoxyguanosine and inorganic tripolyphosphate. [More information is available at EcoCyc: EG10225].
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Genomic context

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Sequence:
NC_000913.3 (179237..180754)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene PF03458 family protein YadS Neighboring gene vitamin B12 ABC transporter periplasmic binding protein Neighboring gene 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Neighboring gene periplasmic serine endoprotease DegP Neighboring gene DNA-binding transcriptional activator CdaR

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Mechanism by which T7 bacteriophage protein Gp1.2 inhibits Escherichia coli dGTPase. Klemm BP, et al. Proc Natl Acad Sci U S A, 2022 Sep 13. PMID 36067314, Free PMC Article
  2. The crystal structure of dGTPase reveals the molecular basis of dGTP selectivity. Barnes CO, et al. Proc Natl Acad Sci U S A, 2019 May 7. PMID 31019074, Free PMC Article
  3. Structure of Escherichia coli dGTP triphosphohydrolase: a hexameric enzyme with DNA effector molecules. Singh D, et al. J Biol Chem, 2015 Apr 17. PMID 25694425, Free PMC Article
  4. A novel mutator of Escherichia coli carrying a defect in the dgt gene, encoding a dGTP triphosphohydrolase. Gawel D, et al. J Bacteriol, 2008 Nov. PMID 18776019, Free PMC Article
  5. Enzymatic cleavage of deoxyguanosine triphosphate to deoxyguanosine and tripolyphosphate. KORNBERG SR, et al. J Biol Chem, 1958 Jul. PMID 13563461

See all (21) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Mechanism by which T7 bacteriophage protein Gp1.2 inhibits Escherichia coli dGTPase.
    Title: Mechanism by which T7 bacteriophage protein Gp1.2 inhibits Escherichia coli dGTPase.
  2. Ec-dGTPase residues at the end of the substrate-binding pocket mimic Watson-Crick interactions providing guanine base specificity.
    Title: The crystal structure of dGTPase reveals the molecular basis of dGTP selectivity.
  3. The enzyme is shown to be a hexameric structure containing regulatory DNA molecules.
    Title: Structure of Escherichia coli dGTP triphosphohydrolase: a hexameric enzyme with DNA effector molecules.
  4. our results suggest that the dgt-dependent pathway for deoxyribose-1-phosphate generation may operate under various cell conditions to provide deoxyribosyl donors.
    Title: The dgt gene of Escherichia coli facilitates thymine utilization in thymine-requiring strains.
  5. Data suggests that alterations of the dGTP pools as well as alterations in the level of some modified dNTP derivatives could affect the fidelity of in vivo DNA replication and, hence, account for the overall mutator effects.
    Title: A novel mutator of Escherichia coli carrying a defect in the dgt gene, encoding a dGTP triphosphohydrolase.
  6. N-terminus verified by Edman degradation on mature peptide
    Title: A unique deoxyguanosine triphosphatase is responsible for the optA1 phenotype of Escherichia coli.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables GTPase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cobalt ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables dGTPase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables dGTPase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables dGTPase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables manganese ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables single-stranded DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in dGTP catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in dGTP catabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within nucleobase-containing small molecule interconversion IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
dGTP triphosphohydrolase
NP_414702.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    179237..180754
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414702.1 dGTP triphosphohydrolase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414702.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A7A6RVI6, A0A828PKA7
    Conserved Domains (1) summary
    PRK04926
    Location:1 → 504
    dgt; deoxyguanosinetriphosphate triphosphohydrolase; Provisional
Nucleotide Protein
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