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fucO L-1,2-propanediol oxidoreductase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947273, updated on 11-Apr-2024

Summary

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Gene symbol
fucO
Gene description
L-1,2-propanediol oxidoreductase
Primary source
ASAP:ABE-0009177
Locus tag
b2799
See related
ECOCYC:EG10351
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2794
Summary
L-1,2-propanediol oxidoreductase is an iron-dependent group III dehydrogenase. [More information is available at EcoCyc: EG10351].
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Genomic context

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Sequence:
NC_000913.3 (2931865..2933013, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene L-serine deaminase II Neighboring gene flap endonuclease Neighboring gene L-fuculose-phosphate aldolase Neighboring gene L-fucose:H(+) symporter

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Substrate scope and selectivity in offspring to an enzyme subjected to directed evolution. Blikstad C, et al. FEBS J, 2014 May. PMID 24673815
  2. Increased furfural tolerance due to overexpression of NADH-dependent oxidoreductase FucO in Escherichia coli strains engineered for the production of ethanol and lactate. Wang X, et al. Appl Environ Microbiol, 2011 Aug. PMID 21685167, Free PMC Article
  3. Crystal structure of an iron-dependent group III dehydrogenase that interconverts L-lactaldehyde and L-1,2-propanediol in Escherichia coli. Montella C, et al. J Bacteriol, 2005 Jul. PMID 15995211, Free PMC Article
  4. Site-directed mutagenesis studies of the metal-binding center of the iron-dependent propanediol oxidoreductase from Escherichia coli. Obradors N, et al. Eur J Biochem, 1998 Nov 15. PMID 9851711
  5. Evolution of an Escherichia coli protein with increased resistance to oxidative stress. Lu Z, et al. J Biol Chem, 1998 Apr 3. PMID 9525938

See all (38) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Analyzed effects of mutations inserted during directed evolution of FucO. Kinetic properties of evolved variants have been determined and the observed differences have been rationalized by modeling the tertiary structures of variant and wild-type enzyme.
    Title: Substrate scope and selectivity in offspring to an enzyme subjected to directed evolution.
  2. Expression of fucO from plasmids was shown to increase furfural tolerance by 50% and to permit the fermentation of 15 mM furfural.
    Title: Increased furfural tolerance due to overexpression of NADH-dependent oxidoreductase FucO in Escherichia coli strains engineered for the production of ethanol and lactate.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables R-lactaldehyde reductase activity IEA
Inferred from Electronic Annotation
more info
  
enables S-lactaldehyde reductase activity IEA
Inferred from Electronic Annotation
more info
  
enables alcohol dehydrogenase (NAD+) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ferrous iron binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables lactaldehyde reductase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables lactaldehyde reductase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within L-fucose catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in fucose catabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in fucose metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glycol catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within propanediol metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within rhamnose catabolic process IEP
Inferred from Expression Pattern
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
L-1,2-propanediol oxidoreductase
NP_417279.2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2931865..2933013 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417279.2 L-1,2-propanediol oxidoreductase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417279.2

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A0H3PMT5, A0A1X3I2X7, A0A1X3JDI6, A0A478L4Q9, A0A829LDK6, A0A8E0FN31, B7LEX9, B7N733, C3SWX2, E1IQN8, E1J8C4, E6BG19, F4V5D8, F4VI03, U9ZLQ9
    Conserved Domains (1) summary
    PRK10624
    Location:1 → 382
    PRK10624; L-1,2-propanediol oxidoreductase; Provisional
Nucleotide Protein
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Gene LinkOut

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