csdA cysteine sulfinate desulfinase [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: csdA
Gene description: cysteine sulfinate desulfinase
Primary source: ASAP:ABE-0009218
Locus tag: b2810
See related: ECOCYC:G7454
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK2806; ygdJ
Summary: csdAE are a multicopy suppressor of iscS. [More information is available at EcoGene: EG13082]. CsdA and CsdE combine to form the CSD sulfur-generating system. [More information is available at EcoCyc: G7454].
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Genomic context

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Sequence:: NC_000913.3 (2943337..2944542)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Herein we present the crystal structure of the oxidized, inactivated CsdE dimer at 2.4 A resolution. The structure sheds light into the activation of the Cys61 nucleophile on its way from a solvent-secluded position in free CsdE to a fully extended conformation in the persulfurated CsdA-CsdE complex.
Title: Insights into the inhibited form of the redox-sensitive SufE-like sulfur acceptor CsdE.
CsdA effects an increase in septation resulting in the formation of coccobacilli at low temperature.
Title: Escherichia coli cold shock protein CsdA effects an increase in septation and the resultant formation of coccobacilli at low temperature.
CsdA interacts with CsdE and a newly discovered protein, called CsdL, resembling E1-like proteins found in ubiquitin-like modification systems.
Title: The CsdA cysteine desulphurase promotes Fe/S biogenesis by recruiting Suf components and participates to a new sulphur transfer pathway by recruiting CsdL (ex-YgdL), a ubiquitin-modifying-like protein.
N-terminus verified by Edman degradation on complete protein
Title: Cysteine sulfinate desulfinase, a NIFS-like protein of Escherichia coli with selenocysteine lyase and cysteine desulfurase activities. Gene cloning, purification, and characterization of a novel pyridoxal enzyme.
CsdA cysteine desulfurase activity is increased 2-fold in the presence of CsdE. Site-directed mutagenesis showed that residue Cys-61 from CsdE acted as an acceptor site for sulfur provided by cysteine desulfurase activity of CsdA
Title: Analysis of the heteromeric CsdA-CsdE cysteine desulfurase, assisting Fe-S cluster biogenesis in Escherichia coli.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables cysteine desulfurase activity	IEA Inferred from Electronic Annotation more info
enables cysteine sulfinate desulfinase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables lyase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables pyridoxal phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables pyridoxal phosphate binding	IEA Inferred from Electronic Annotation more info
enables selenocysteine lyase activity	IEA Inferred from Electronic Annotation more info
enables sulfurtransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in L-cysteine catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in cysteine metabolic process	IEA Inferred from Electronic Annotation more info
involved_in iron-sulfur cluster assembly	IEA Inferred from Electronic Annotation more info
involved_in selenocysteine catabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within sulfur amino acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in sulfur compound transport	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of L-cysteine desulfurase complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: cysteine sulfinate desulfinase

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

2943337..2944542

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417290.1 cysteine sulfinate desulfinase [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417290.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A2X1NA06

Conserved Domains (1) summary

PRK10874
Location:1 → 401

PRK10874; cysteine desulfurase CsdA