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ushA 5'-nucleotidase/UDP-sugar hydrolase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947331, updated on 4-Feb-2024

Summary

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Gene symbol
ushA
Gene description
5'-nucleotidase/UDP-sugar hydrolase
Primary source
ASAP:ABE-0001668
Locus tag
b0480
See related
ECOCYC:EG11060
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0474
Summary
UshA has highly efficient CDP-alcohol hydrolase activity (Alves-Pereira, 2008). [More information is available at EcoGene: EG11060]. Salmonella has a different gene and enzyme, ushB. [More information is available at EcoCyc: EG11060].
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Genomic context

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Sequence:
NC_000913.3 (504914..506566)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative transporter YbaL Neighboring gene fosmidomycin efflux pump Neighboring gene Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK Neighboring gene ncRNA Neighboring gene TraB family protein YbaP

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Ecto-5'-nucleotidase: Structure function relationships. Sträter N. Purinergic Signal, 2006 Jun. PMID 18404474, Free PMC Article
  2. A large hinge bending domain rotation is necessary for the catalytic function of Escherichia coli 5'-nucleotidase. Schultz-Heienbrok R, et al. Biochemistry, 2005 Feb 22. PMID 15709736
  3. The role of the intracellular inhibitor of periplasmic UDP-sugar hydrolase (5'-nucleotidase) in Escherichia coli: cytoplasmic localisation of 5'-nucleotidase is conditionally lethal. Innes D, et al. J Basic Microbiol, 2001. PMID 11802543
  4. Kinetic characteristics of nucleoside mono-, di- and triphosphatase activities of the periplasmic 5'-nucleotidase of Escherichia coli. García L, et al. Comp Biochem Physiol B Biochem Mol Biol, 1997 May. PMID 9180021
  5. Characterization of the ush gene of Escherichia coli and its protein products. Burns DM, et al. Gene, 1983 Nov. PMID 6363215

See all (28) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. CDP-alcohol hydrolase activity was added to the spectrum of UshA enzyme activities.
    Title: CDP-alcohol hydrolase, a very efficient activity of the 5'-nucleotidase/UDP-sugar hydrolase encoded by the ushA gene of Yersinia intermedia and Escherichia coli.
  2. An ushA aphA double knockout mutant was shown to be unable to grow on purine nucleotides as the sole carbon source.
    Title: Complete deficiency of 5'-nucleotidase activity in Escherichia coli leads to loss of growth on purine nucleotides but not of their excretion.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
  4. Two variants of Escherichia coli 5'-nucleotidase with disulfide bridges that are engineered to link the two domains of the protein are used to demonstrate that a large domain rotation is required for the catalytic mechanism of the enzyme.
    Title: A large hinge bending domain rotation is necessary for the catalytic function of Escherichia coli 5'-nucleotidase.
Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 5'-nucleotidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 5'-nucleotidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 5'-nucleotidase activity IEA
Inferred from Electronic Annotation
more info
  
enables UDP-sugar diphosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables UDP-sugar diphosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables UDP-sugar diphosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables XMP 5'-nucleosidase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on ester bonds IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotidase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in nucleotide catabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in outer membrane-bounded periplasmic space IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in outer membrane-bounded periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in outer membrane-bounded periplasmic space IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in periplasmic space IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
5'-nucleotidase/UDP-sugar hydrolase
NP_415013.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    504914..506566
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415013.1 5'-nucleotidase/UDP-sugar hydrolase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415013.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A7L5V1U8
    Conserved Domains (1) summary
    PRK09558
    Location:1 → 550
    ushA; bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor; Reviewed
Nucleotide Protein
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