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lysS lysine--tRNA ligase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947372, updated on 2-May-2024

Summary

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Gene symbol
lysS
Gene description
lysine--tRNA ligase
Primary source
ASAP:ABE-0009486
Locus tag
b2890
See related
ECOCYC:EG10552
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
asuD; ECK2885; herC
Summary
Mutants have reduced OM vesiculation (McBroom, 2006). [More information is available at EcoGene: EG10552]. The lysyl-tRNA synthetase LysS is a member of the family of aminoacyl tRNA synthetases, which interpret the genetic code by covalently linking amino acids to their specific tRNA molecules. [More information is available at EcoCyc: EG10552].
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Genomic context

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Sequence:
NC_000913.3 (3033657..3035174, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene uncharacterized protein YqfG Neighboring gene protein YqfH Neighboring gene isopentenyl-diphosphate Delta-isomerase Neighboring gene peptide chain release factor RF2 Neighboring gene ssDNA-specific exonuclease RecJ

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Discrimination of cognate and noncognate substrates at the active site of class II lysyl-tRNA synthetase. Ataide SF, et al. Biochemistry, 2004 Sep 21. PMID 15362869
  2. Structural studies of lysyl-tRNA synthetase: conformational changes induced by substrate binding. Onesti S, et al. Biochemistry, 2000 Oct 24. PMID 11041850
  3. Substrate recognition by class I lysyl-tRNA synthetases: a molecular basis for gene displacement. Ibba M, et al. Proc Natl Acad Sci U S A, 1999 Jan 19. PMID 9892648, Free PMC Article
  4. tRNA anticodon recognition and specification within subclass IIb aminoacyl-tRNA synthetases. Commans S, et al. J Mol Biol, 1998 May 15. PMID 9614943
  5. Solution structure of the anticodon-binding domain of Escherichia coli lysyl-tRNA synthetase and studies of its interaction with tRNA(Lys). Commans S, et al. J Mol Biol, 1995 Oct 13. PMID 7473706

See all (39) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on mature peptide
    Title: Homology of lysS and lysU, the two Escherichia coli genes encoding distinct lysyl-tRNA synthetase species.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables aminoacyl-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables lysine-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables lysine-tRNA ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables lysine-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables lysine-tRNA ligase activity IGI
Inferred from Genetic Interaction
more info
 PubMed 
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in lysyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in lysyl-tRNA aminoacylation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within lysyl-tRNA aminoacylation IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within tRNA aminoacylation for protein translation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane HDA PubMed 

General protein information

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Preferred Names
lysine--tRNA ligase
NP_417366.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3033657..3035174 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417366.1 lysine--tRNA ligase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417366.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8H9JI30, A0A8S7RVU4
    Conserved Domains (1) summary
    PRK00484
    Location:14 → 503
    lysS; lysyl-tRNA synthetase; Reviewed
Nucleotide Protein
Heading Accession and Version

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