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gss fused glutathionylspermidine amidase/glutathionylspermidine synthetase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947474, updated on 23-Nov-2023

Summary

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Gene symbol
gss
Gene description
fused glutathionylspermidine amidase/glutathionylspermidine synthetase
Primary source
ASAP:ABE-0009804
Locus tag
b2988
See related
ECOCYC:EG12882
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2982; gsp
Summary
gsp encodes a bifunctional protein with two domains: an N-terminal GSP amidase domain and a C-terminal GSP synthetase domain. [More information is available at EcoCyc: EG12882].
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Genomic context

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Sequence:
NC_000913.3 (3136663..3138522, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative ATP-binding protein YghT Neighboring gene metal phosphate:H(+) symporter PitB Neighboring gene disulfide reductase/organic hydroperoxide reductase Neighboring gene hydrogenase maturation factor HybG

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Protein S-thiolation by Glutathionylspermidine (Gsp): the role of Escherichia coli Gsp synthetASE/amidase in redox regulation. Chiang BY, et al. J Biol Chem, 2010 Aug 13. PMID 20530482, Free PMC Article
  2. Evidence for a glutathionyl-enzyme intermediate in the amidase activity of the bifunctional glutathionylspermidine synthetase/amidase from Escherichia coli. Lin CH, et al. Biochemistry, 1997 Dec 2. PMID 9398217
  3. Aldehyde and phosphinate analogs of glutathione and glutathionylspermidine: potent, selective binding inhibitors of the E. coli bifunctional glutathionylspermidine synthetase/amidase. Lin CH, et al. Chem Biol, 1997 Nov. PMID 9384533
  4. Design, synthesis, and biochemical evaluation of phosphonate and phosphonamidate analogs of glutathionylspermidine as inhibitors of glutathionylspermidine synthetase/amidase from Escherichia coli. Chen S, et al. J Med Chem, 1997 Nov 7. PMID 9371250
  5. Dissection of glutathionylspermidine synthetase/amidase from Escherichia coli into autonomously folding and functional synthetase and amidase domains. Kwon DS, et al. J Biol Chem, 1997 Jan 24. PMID 8999955

See all (18) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Results describe two crystal structures of glutathionylspermidine amidase, GspA and GspSA, both with the C59A mutation and bound with the substrate, Gsp.
    Title: Structure and mechanism of Escherichia coli glutathionylspermidine amidase belonging to the family of cysteine; histidine-dependent amidohydrolases/peptidases.
  2. The hypersensitivities of GspSA and GspSA/glutaredoxin null mutants to H(2)O(2) support the idea that GspSA and glutaredoxin act synergistically to regulate the redox environment of E. coli.
    Title: Protein S-thiolation by Glutathionylspermidine (Gsp): the role of Escherichia coli Gsp synthetASE/amidase in redox regulation.
  3. Five crystal structures of Escherichia coli GspS in complex with substrate, product or inhibitor, are reported.
    Title: Dual binding sites for translocation catalysis by Escherichia coli glutathionylspermidine synthetase.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables glutathionylspermidine amidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glutathionylspermidine amidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glutathionylspermidine amidase activity IEA
Inferred from Electronic Annotation
more info
  
enables glutathionylspermidine synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glutathionylspermidine synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glutathionylspermidine synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in glutathione metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in spermidine metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
fused glutathionylspermidine amidase/glutathionylspermidine synthetase
NP_417462.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3136663..3138522 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417462.1 fused glutathionylspermidine amidase/glutathionylspermidine synthetase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417462.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    D3GVP7, F4VIY6
    Conserved Domains (1) summary
    PRK10507
    Location:1 → 618
    PRK10507; bifunctional glutathionylspermidine amidase/glutathionylspermidine synthetase; Provisional
Nucleotide Protein
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