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mdaB NADPH:quinone oxidoreductase MdaB [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947512, updated on 6-Oct-2023

Summary

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Gene symbol
mdaB
Gene description
NADPH:quinone oxidoreductase MdaB
Primary source
ASAP:ABE-0009946
Locus tag
b3028
See related
ECOCYC:EG12656
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3019; mda66
Summary
MdaB overexpression results in resistance to cytotoxic drugs including adriamycin, etoposide and the tumoricidal agent DMP 840. [More information is available at EcoGene: EG12656]. The MdaB quinone reductase is specific for NADPH and is most active with quinone derivatives and ferricyanide . [More information is available at EcoCyc: EG12656].
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Genomic context

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Sequence:
NC_000913.3 (3172530..3173111)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional activator QseB Neighboring gene sensor histidine kinase QseC Neighboring gene DUF2645 domain-containing inner membrane protein YgiZ Neighboring gene putative quinol monooxygenase YgiN Neighboring gene DNA topoisomerase IV subunit B

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. MdaB from Escherichia coli: cloning, purification, crystallization and preliminary X-ray analysis. Adams MA, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2005 Feb 1. PMID 16511004, Free PMC Article
  2. Potential involvement of several nitroreductases in metronidazole resistance in Helicobacter pylori. Jorgensen MA, et al. Arch Biochem Biophys, 2001 Aug 15. PMID 11488591
  3. Characterization of FMN-dependent NADH-quinone reductase induced by menadione in Escherichia coli. Hayashi M, et al. Biochim Biophys Acta, 1990 Aug 17. PMID 2118386
  4. A general genetic approach in Escherichia coli for determining the mechanism(s) of action of tumoricidal agents: application to DMP 840, a tumoricidal agent. Chatterjee PK, et al. Proc Natl Acad Sci U S A, 1995 Sep 12. PMID 7568050, Free PMC Article
  5. Piecing together the structure-function puzzle: experiences in structure-based functional annotation of hypothetical proteins. Adams MA, et al. Proteomics, 2007 Aug. PMID 17639604

See all (13) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on mature peptide
    Title: NADPH-specific quinone reductase is induced by 2-methylene-4-butyrolactone in Escherichia coli.
  2. The three-dimensional crystal structure of apo-MdaB reported in this study exhibits significant structural similarity to a number of flavoproteins, including the mammalian DT-diaphorases.
    Title: Modulator of drug activity B from Escherichia coli: crystal structure of a prokaryotic homologue of DT-diaphorase.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables NADPH dehydrogenase (quinone) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables NADPH dehydrogenase (quinone) activity IEA
Inferred from Electronic Annotation
more info
  
enables flavin adenine dinucleotide binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
NADPH:quinone oxidoreductase MdaB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3172530..3173111
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417500.1 NADPH:quinone oxidoreductase MdaB [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417500.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6C9FIH4, A0A7A6U6A5
    Conserved Domains (1) summary
    COG2249
    Location:3 → 193
    MdaB; Putative NADPH-quinone reductase (modulator of drug activity B) [General function prediction only]
Nucleotide Protein
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