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tolC outer membrane channel TolC [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947521, updated on 4-Apr-2024

Summary

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Gene symbol
tolC
Gene description
outer membrane channel TolC
Primary source
ASAP:ABE-0009965
Locus tag
b3035
See related
ECOCYC:EG11009
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
colE1-i; ECK3026; mtcB; mukA; refI; toc; weeA
Summary
TolC, MdtQ, MdtP and CusC are paralogs. [More information is available at EcoGene: EG11009]. TolC is an outer membrane porin involved in the efflux of several hydrophobic and amphipathic molecules. [More information is available at EcoCyc: EG11009].
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Genomic context

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Sequence:
NC_000913.3 (3178115..3179596)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF1249 domain-containing protein YqiB Neighboring gene ADP-sugar pyrophosphatase Neighboring gene DUF1190 domain-containing protein YgiB Neighboring gene putative acid--amine ligase YgiC

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The Antibiotic Efflux Protein TolC Is a Highly Evolvable Target under Colicin E1 or TLS Phage Selection. Tamer YT, et al. Mol Biol Evol, 2021 Sep 27. PMID 34175926, Free PMC Article
  2. Biophysical characterization of E. coli TolC interaction with the known blocker hexaamminecobalt. Gilardi A, et al. Biochim Biophys Acta Gen Subj, 2017 Nov. PMID 28746830
  3. Enhanced Efflux Activity Facilitates Drug Tolerance in Dormant Bacterial Cells. Pu Y, et al. Mol Cell, 2016 Apr 21. PMID 27105118, Free PMC Article
  4. Dynamic interplay of multidrug transporters with TolC for isoprenol tolerance in Escherichia coli. Wang C, et al. Sci Rep, 2015 Nov 13. PMID 26563610, Free PMC Article
  5. Structure-function of cyanobacterial outer-membrane protein, Slr1270: homolog of Escherichia coli drug export/colicin import protein, TolC. Agarwal R, et al. FEBS Lett, 2014 Nov 3. PMID 25218435, Free PMC Article

See all (220) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The Antibiotic Efflux Protein TolC Is a Highly Evolvable Target under Colicin E1 or TLS Phage Selection.
    Title: The Antibiotic Efflux Protein TolC Is a Highly Evolvable Target under Colicin E1 or TLS Phage Selection.
  2. In situ structure of the AcrAB-TolC efflux pump at subnanometer resolution.
    Title: In situ structure of the AcrAB-TolC efflux pump at subnanometer resolution.
  3. Pyridylpiperazine-based allosteric inhibitors of RND-type multidrug efflux pumps.
    Title: Pyridylpiperazine-based allosteric inhibitors of RND-type multidrug efflux pumps.
  4. Extreme Acid Modulates Fitness Trade-Offs of Multidrug Efflux Pumps MdtEF-TolC and AcrAB-TolC in Escherichia coli K-12.
    Title: Extreme Acid Modulates Fitness Trade-Offs of Multidrug Efflux Pumps MdtEF-TolC and AcrAB-TolC in Escherichia coli K-12.
  5. Outer Membrane Channel Protein TolC Regulates Escherichia coli K12 Sensitivity to Plantaricin BM-1 via the CpxR/CpxA Two-Component Regulatory System.
    Title: Outer Membrane Channel Protein TolC Regulates Escherichia coli K12 Sensitivity to Plantaricin BM-1 via the CpxR/CpxA Two-Component Regulatory System.
  6. Structural characterization of the EmrAB-TolC efflux complex from E. coli.
    Title: Structural characterization of the EmrAB-TolC efflux complex from E. coli.
  7. EntE, EntS and TolC synergistically contributed to the pathogenesis of APEC strain E058.
    Title: EntE, EntS and TolC synergistically contributed to the pathogenesis of APEC strain E058.
  8. Discovery of multidrug efflux pump inhibitors with a novel chemical scaffold.
    Title: Discovery of multidrug efflux pump inhibitors with a novel chemical scaffold.
  9. AcrA, the membrane fusion protein, connects the inner-membrane-bound AcrB to the outer-membrane-bound TolC to form the multidrug efflux pump.
    Title: Conformational Dynamics of AcrA Govern Multidrug Efflux Pump Assembly.
  10. In the presence of translation-inhibiting antibiotics, resistance acquisition depends on the AcrAB-TolC multidrug efflux pump, because it reduces tetracycline concentrations in the cell.
    Title: Role of AcrAB-TolC multidrug efflux pump in drug-resistance acquisition by plasmid transfer.
Submit: New GeneRIF Correction See all GeneRIFs (52)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables bile acid transmembrane transporter activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables efflux transmembrane transporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables efflux transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables efflux transmembrane transporter activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables monoatomic ion channel activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables porin activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables porin activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within bile acid and bile salt transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within enterobactin transport IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in monoatomic ion transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in response to antibiotic IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to antibiotic IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within response to organic cyclic compound IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within response to toxic substance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within response to xenobiotic stimulus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in xenobiotic detoxification by transmembrane export across the cell outer membrane EXP
Inferred from Experiment
more info
 PubMed 
involved_in xenobiotic detoxification by transmembrane export across the cell outer membrane IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in xenobiotic detoxification by transmembrane export across the cell outer membrane NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in xenobiotic detoxification by transmembrane export across the plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of MacAB-TolC complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of MacAB-TolC complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cell outer membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cell outer membrane IEA
Inferred from Electronic Annotation
more info
  
part_of efflux pump complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of efflux pump complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of efflux pump complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of efflux pump complex NAS
Non-traceable Author Statement
more info
 PubMed 
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in outer membrane IEA
Inferred from Electronic Annotation
more info
  
located_in outer membrane-bounded periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in periplasmic side of plasma membrane NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
outer membrane channel TolC

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3178115..3179596
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417507.2 outer membrane channel TolC [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417507.2

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0AA86ZGL3
    Conserved Domains (1) summary
    PRK09465
    Location:1 → 451
    tolC; outer membrane channel protein; Reviewed
Nucleotide Protein
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