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btuF vitamin B12 ABC transporter periplasmic binding protein [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947574, updated on 4-Apr-2024

Summary

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Gene symbol
btuF
Gene description
vitamin B12 ABC transporter periplasmic binding protein
Primary source
ASAP:ABE-0000542
Locus tag
b0158
See related
ECOCYC:EG12334
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0157; yadT
Summary
Uncleaved precursor N-terminus was also sequenced, confirming this start site of translation. about 80% of the precursors had the initial Met cleaved. [More information is available at EcoGene: EG12334]. BtuF is the periplasmic vitamin B12 binding protein that delivers cobalamin to the ABC transporter BtuCD. [More information is available at EcoCyc: EG12334].
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Genomic context

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Sequence:
NC_000913.3 (177662..178462, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YadW Neighboring gene iron-sulfur cluster insertion protein ErpA Neighboring gene PF03458 family protein YadS Neighboring gene 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Neighboring gene dGTP triphosphohydrolase Neighboring gene periplasmic serine endoprotease DegP

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Study on the mechanism of the BtuF periplasmic-binding protein for vitamin B12. Liu M, et al. Biophys Chem, 2008 Jun. PMID 18358587
  2. Opening and closing motions in the periplasmic vitamin B12 binding protein BtuF. Kandt C, et al. Biochemistry, 2006 Nov 7. PMID 17073449
  3. Crystal structures of the BtuF periplasmic-binding protein for vitamin B12 suggest a functionally important reduction in protein mobility upon ligand binding. Karpowich NK, et al. J Biol Chem, 2003 Mar 7. PMID 12468528
  4. A new class of cobalamin transport mutants (btuF) provides genetic evidence for a periplasmic binding protein in Salmonella typhimurium. Van Bibber M, et al. J Bacteriol, 1999 Sep. PMID 10464235, Free PMC Article
  5. TonB interacts with BtuF, the Escherichia coli periplasmic binding protein for cyanocobalamin. James KJ, et al. Biochemistry, 2009 Oct 6. PMID 19708689

See all (30) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The periplasmic soluble binding protein BtuF binds the vitamin B12 and the transmembrane and ATPase domains BtuCD mediate the translocation.
    Title: Single-molecule visualization of conformational changes and substrate transport in the vitamin B(12) ABC importer BtuCD-F.
  2. study used molecular dynamics simulations to investigate the atomic-level mechanism of conformational coupling in the ABC transporter BtuCD-F
    Title: Release of Entropic Spring Reveals Conformational Coupling Mechanism in the ABC Transporter BtuCD-F.
  3. Data suggest that BtuF does not discriminate between, or impose, asymmetric conformations of BtuC/BtuD complex. Data confirm conformational disorder observed in BtuC/BtuD/BtuF crystals.
    Title: Asymmetric states of vitamin B₁₂ transporter BtuCD are not discriminated by its cognate substrate binding protein BtuF.
  4. N-terminus verified by Edman degradation on mature peptide
    Title: Identification of the periplasmic cobalamin-binding protein BtuF of Escherichia coli.
  5. The 2.6 angstrom crystal structure of a complex BtuCD-F reveals substantial conformational changes as compared with the previously reported structures of BtuCD and BtuF
    Title: Asymmetry in the structure of the ABC transporter-binding protein complex BtuCD-BtuF.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables cobalamin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables cobalamin binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within cobalamin transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cobalamin transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cobalamin transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cobalamin transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of cobalamin transport complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in outer membrane-bounded periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in periplasmic space IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
vitamin B12 ABC transporter periplasmic binding protein
NP_414700.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    177662..178462 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414700.1 vitamin B12 ABC transporter periplasmic binding protein [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414700.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A192CIC8, D6J621
    Conserved Domains (1) summary
    PRK03379
    Location:7 → 266
    PRK03379; vitamin B12-transporter protein BtuF; Provisional
Nucleotide Protein
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