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gapA glyceraldehyde-3-phosphate dehydrogenase A [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947679, updated on 6-Oct-2023

Summary

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Gene symbol
gapA
Gene description
glyceraldehyde-3-phosphate dehydrogenase A
Primary source
ASAP:ABE-0005920
Locus tag
b1779
See related
ECOCYC:EG10367
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1777; gad; gap1
Summary
A gapA mutant is not in the Keio collection; gapA mutants were not expected to grow on the medium used to attempt to isolate them; gapA is not an essential gene (Baba, 2006). [More information is available at EcoGene: EG10367]. Escherichia coli is unusual in having two glyceraldehyde-3-phosphate dehydrogenases. [More information is available at EcoCyc: EG10367].
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Genomic context

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Sequence:
NC_000913.3 (1862771..1863766)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF1315 domain-containing protein YeaC Neighboring gene methionine sulfoxide reductase B Neighboring gene putative aldose 1-epimerase YeaD Neighboring gene methylglyoxal reductase YeaE

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Characterization and structure of glyceraldehyde-3-phosphate dehydrogenase type 1 from Escherichia coli. Zhang L, et al. Acta Crystallogr F Struct Biol Commun, 2020 Sep 1. PMID 32880588, Free PMC Article
  2. NAD+-dependent post-translational modification of Escherichia coli glyceraldehyde-3-phosphate dehydrogenase. Aguilera L, et al. Int Microbiol, 2009 Sep. PMID 19784925
  3. Structure of insoluble rat sperm glyceraldehyde-3-phosphate dehydrogenase (GAPDH) via heterotetramer formation with Escherichia coli GAPDH reveals target for contraceptive design. Frayne J, et al. J Biol Chem, 2009 Aug 21. PMID 19542219, Free PMC Article
  4. Role of secreted glyceraldehyde-3-phosphate dehydrogenase in the infection mechanism of enterohemorrhagic and enteropathogenic Escherichia coli: interaction of the extracellular enzyme with human plasminogen and fibrinogen. Egea L, et al. Int J Biochem Cell Biol, 2007. PMID 17449317
  5. The strong efficiency of the Escherichia coli gapA P1 promoter depends on a complex combination of functional determinants. Thouvenot B, et al. Biochem J, 2004 Oct 15. PMID 15250823, Free PMC Article

See all (56) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Characterization and structure of glyceraldehyde-3-phosphate dehydrogenase type 1 from Escherichia coli.
    Title: Characterization and structure of glyceraldehyde-3-phosphate dehydrogenase type 1 from Escherichia coli.
  2. The authors report that GAPDH of these pathogens is ADP-ribosylated either in the cytoplasm or in the extracellular medium. GAPDH catalyzes its own modification, which involves Cys-149 at the active site.
    Title: NAD+-dependent post-translational modification of Escherichia coli glyceraldehyde-3-phosphate dehydrogenase.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: Direct detection of potential selenium delivery proteins by using an Escherichia coli strain unable to incorporate selenium from selenite into proteins.
  4. Data indicate that exported glyceraldehyde-3-phosphate dehydrogenase may act as a virulence factor which could contribute to bacterial pathogenesis.
    Title: Role of secreted glyceraldehyde-3-phosphate dehydrogenase in the infection mechanism of enterohemorrhagic and enteropathogenic Escherichia coli: interaction of the extracellular enzyme with human plasminogen and fibrinogen.
  5. promoter gapA P1 is a very efficient promoter sharing common properties with both extended -10 and non-extended -10 promoters
    Title: The strong efficiency of the Escherichia coli gapA P1 promoter depends on a complex combination of functional determinants.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables NAD binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables NAD binding IEA
Inferred from Electronic Annotation
more info
  
enables NAD binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables NADP binding IEA
Inferred from Electronic Annotation
more info
  
enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in glucose metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in glycolytic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glycolytic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane HDA PubMed 

General protein information

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Preferred Names
glyceraldehyde-3-phosphate dehydrogenase A
NP_416293.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1862771..1863766
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416293.1 glyceraldehyde-3-phosphate dehydrogenase A [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416293.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A793CDN0
    Conserved Domains (1) summary
    PRK15425
    Location:1 → 331
    gapA; glyceraldehyde-3-phosphate dehydrogenase
Nucleotide Protein
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