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lptA lipopolysaccharide transport system protein LptA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947920, updated on 23-Nov-2023

Summary

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Gene symbol
lptA
Gene description
lipopolysaccharide transport system protein LptA
Primary source
ASAP:ABE-0010510
Locus tag
b3200
See related
ECOCYC:G7665
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3189; yhbN
Summary
LptA depletion results in rifampicin/bile salts sensitivity, indicative of an outer membrane integrity defect. [More information is available at EcoGene: EG12618]. LptA is a periplasmic binding component of the LptABC ABC transporter. [More information is available at EcoCyc: G7665].
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Genomic context

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Sequence:
NC_000913.3 (3343380..3343937)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene D-arabinose 5-phosphate isomerase KdsD Neighboring gene 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC Neighboring gene lipopolysaccharide transport system protein LptC Neighboring gene lipopolysaccharide transport system ATP binding protein LptB Neighboring gene RNA polymerase sigma factor RpoN

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Mutation and Suppressor Analysis of the Essential Lipopolysaccharide Transport Protein LptA Reveals Strategies To Overcome Severe Outer Membrane Permeability Defects in Escherichia coli. Falchi FA, et al. J Bacteriol, 2018 Jan 15. PMID 29109183, Free PMC Article
  2. Lipopolysaccharide binding to the periplasmic protein LptA. Schultz KM, et al. Protein Sci, 2017 Aug. PMID 28419595, Free PMC Article
  3. An induced folding process characterizes the partial-loss of function mutant LptAI36D in its interactions with ligands. Santambrogio C, et al. Biochim Biophys Acta, 2015 Oct. PMID 26123264
  4. LptA assembles into rod-like oligomers involving disorder-to-order transitions. Santambrogio C, et al. J Am Soc Mass Spectrom, 2013 Oct. PMID 23897621
  5. Concentration-dependent oligomerization and oligomeric arrangement of LptA. Merten JA, et al. Protein Sci, 2012 Feb. PMID 22109962, Free PMC Article

See all (33) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Characterized interactions and binding of lipopolysaccharide transport system proteins LptA and LptC thru dimerization and point mutation studies.
    Title: Disruption of the E. coli LptC dimerization interface and characterization of lipopolysaccharide and LptA binding to monomeric LptC.
  2. characterization of a viable quadruple lptA mutant impaired in the assembly of the Lpt machinery, although it retains the ability to bind LPS; although viable, the quadruple lptA mutant disrupts outer membrane (OM) asymmetry and severely impairs the OM permeability barrier; analysis of 2 independent suppressors revealed different strategies adopted by the cell to overcome defects in LPS biogenesis
    Title: Mutation and Suppressor Analysis of the Essential Lipopolysaccharide Transport Protein LptA Reveals Strategies To Overcome Severe Outer Membrane Permeability Defects in Escherichia coli.
  3. LptA molecular structure is affected by lipopolysaccharides binding.
    Title: Lipopolysaccharide binding to the periplasmic protein LptA.
  4. An induced folding process characterizes the partial-loss of function mutant LptAI36D in its interactions with lipopolysaccharide constituent ligands.
    Title: An induced folding process characterizes the partial-loss of function mutant LptAI36D in its interactions with ligands.
  5. Studied the assembly and architecture of LptA oligomers.
    Title: LptA assembles into rod-like oligomers involving disorder-to-order transitions.
  6. results suggest that LptA operate in the LPS assembly pathway and, together with other as-yet-unidentified components, could be part of a complex devoted to the transport of LPS from the periplasmic surface of the IM to the OM.
    Title: Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli.
  7. LptA binds structurally diverse lipopolysaccharide (LPS) substrates in vitro and demonstrate that it interacts specifically with the lipid A domain of LPS
    Title: The LptA protein of Escherichia coli is a periplasmic lipid A-binding protein involved in the lipopolysaccharide export pathway.
  8. N-terminus verified by Edman degradation on mature peptide
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables glycolipid transfer activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glycolipid transfer activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables lipopolysaccharide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in Gram-negative-bacterium-type cell outer membrane assembly IEA
Inferred from Electronic Annotation
more info
  
involved_in glycolipid transport IEA
Inferred from Electronic Annotation
more info
  
involved_in lipopolysaccharide transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in lipopolysaccharide transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within lipopolysaccharide transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cell outer membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cell outer membrane IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in outer membrane-bounded periplasmic space IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in outer membrane-bounded periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in periplasmic space IEA
Inferred from Electronic Annotation
more info
  
part_of transporter complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
lipopolysaccharide transport system protein LptA
NP_417667.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3343380..3343937
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417667.1 lipopolysaccharide transport system protein LptA [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417667.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9ADM4, A0A5B9APM3, A0A5D8MTI4
    Conserved Domains (1) summary
    PRK10894
    Location:5 → 185
    PRK10894; lipopolysaccharide ABC transporter substrate-binding protein LptA
Nucleotide Protein
Heading Accession and Version

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