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gadA glutamate decarboxylase A [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948027, updated on 4-Apr-2024

Summary

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Gene symbol
gadA
Gene description
glutamate decarboxylase A
Primary source
ASAP:ABE-0011490
Locus tag
b3517
See related
ECOCYC:EG50009
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3502; gadS
Summary
RpoS regulon. [More information is available at EcoGene: EG50009]. GadA, a glutamate decarboxylase enzyme, is part of the glutamate-dependent acid resistance system 2 (AR2) which confers resistance to extreme acid conditions. [More information is available at EcoCyc: EG50009].
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Genomic context

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Sequence:
NC_000913.3 (3666180..3667580, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene DNA-binding transcriptional dual regulator GadX Neighboring gene cytochrome c peroxidase Neighboring gene cytoplasmic trehalase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structure of Escherichia coli glutamate decarboxylase (GADalpha) in complex with glutarate at 2.05 angstroms resolution. Dutyshev DI, et al. Acta Crystallogr D Biol Crystallogr, 2005 Mar. PMID 15735332
  2. The critical structural role of a highly conserved histidine residue in group II amino acid decarboxylases. Capitani G, et al. FEBS Lett, 2003 Nov 6. PMID 14596911
  3. Structure of glutamate decarboxylase from E. coli: spectral studies. Bazhulina NP, et al. J Biomol Struct Dyn, 2002 Jun. PMID 12023802
  4. [Glutamate decarboxylase from Escherichia coli: expression of gadA gene, purification and properties of GADalpha]. Shul'ga AA, et al. Mol Biol (Mosk), 1999 Jul-Aug. PMID 10546205
  5. [Study of the quaternary structure of glutamate carboxylase from Escherichia coli]. Sukhareva BS, et al. Mol Biol (Mosk), 1994 Nov-Dec. PMID 7885340

See all (44) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that Corynebacterium glutamicum expressing E. coli glutamate decarboxylase (GAD) mutant under the strong PH36 promoter could produce GABA 17-fold higher than that obtained by C. glutamicum expressing wild-type E. coli GAD.
    Title: Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum by expressing glutamate decarboxylase active in expanded pH range.
  2. activation of gadA transcription requires a GAD box and binding of an RcsB/GadE heterodimer.
    Title: Acid stress response in Escherichia coli: mechanism of regulation of gadA transcription by RcsB and GadE.
  3. N-terminus verified by Edman degradation on complete protein
    Title: Acid- and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis.
  4. H-NS directly inhibits gadA and gadX transcription and, by controlling the intracellular level of the activator GadX, indirectly affects the expression of the whole gad system
    Title: Antagonistic role of H-NS and GadX in the regulation of the glutamate decarboxylase-dependent acid resistance system in Escherichia coli.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables carbon-carbon lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables carboxy-lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables glutamate decarboxylase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glutamate decarboxylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glutamate decarboxylase activity IEA
Inferred from Electronic Annotation
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables pyridoxal phosphate binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in carboxylic acid metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in glutamate catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in glutamate metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within intracellular pH elevation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in membrane HDA PubMed 

General protein information

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Preferred Names
glutamate decarboxylase A
NP_417974.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3666180..3667580 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417974.1 glutamate decarboxylase A [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417974.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A827QC53, A0A828NQW2, A0AA88JKA9, A5YKF4, A5YKF6
    Conserved Domains (1) summary
    TIGR01788
    Location:16 → 449
    Glu-decarb-GAD; glutamate decarboxylase
Nucleotide Protein
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