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dctA C4 dicarboxylate/orotate:H(+) symporter [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948039, updated on 4-Apr-2024

Summary

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Gene symbol
dctA
Gene description
C4 dicarboxylate/orotate:H(+) symporter
Primary source
ASAP:ABE-0011527
Locus tag
b3528
See related
ECOCYC:EG20044
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3513
Summary
DctA is required for dicarboxylate transport . [More information is available at EcoCyc: EG20044].
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Genomic context

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Sequence:
NC_000913.3 (3682161..3683447, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 2-dehydro-3-deoxygluconokinase Neighboring gene peptidase M16 family protein YhjJ Neighboring gene c-di-GMP phosphodiesterase PdeK Neighboring gene cellulose synthase outer membrane channel Neighboring gene endo-1,4-D-glucanase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Genetic control of the uptake of C(4)-dicarboxylic acids by Escherichia coli. Kay WW, et al. FEBS Lett, 1969 Apr. PMID 11946977
  2. CRP interacts with promoter-bound sigma54 RNA polymerase and blocks transcriptional activation of the dctA promoter. Wang YP, et al. EMBO J, 1998 Feb 2. PMID 9451003, Free PMC Article
  3. Utilization of orotate as a pyrimidine source by Salmonella typhimurium and Escherichia coli requires the dicarboxylate transport protein encoded by dctA. Baker KE, et al. J Bacteriol, 1996 Dec. PMID 8955389, Free PMC Article
  4. Transport of succinate in Escherichia coli. II. Characteristics of uptake and energy coupling with transport in membrane preparations. Rayman MK, et al. J Biol Chem, 1972 Oct 10. PMID 4568614
  5. Genetic analysis of mutants of Escherichia coli defective in dicarboxylate transport. Lo TC, et al. Mol Gen Genet, 1975 Oct 22. PMID 1107801

See all (41) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. In this work, it is shown that DctA is required for aerobic growth with L-aspartate as a carbon source.
    Title: DcuA of aerobically grown Escherichia coli serves as a nitrogen shuttle (L-aspartate/fumarate) for nitrogen uptake.
  2. The relationship of absolute contents of DcuS, DcuB, and DctA to the functional state of DcuS revealed their contribution to the control of DcuS-DcuR in vivo, which was not accessible by other approaches, leading to a revision of previous models.
    Title: Cellular Concentrations of the Transporters DctA and DcuB and the Sensor DcuS of Escherichia coli and the Contributions of Free and Complexed DcuS to Transcriptional Regulation by DcuR.
  3. DcuS is required for location of DctA and DcuR at the poles and formation of tripartite DctA/DcuS/DcuR sensor/regulator complexes.
    Title: Polar localization of a tripartite complex of the two-component system DcuS/DcuR and the transporter DctA in Escherichia coli depends on the sensor kinase DcuS.
  4. C4-dicarboxylate sensing by DctA/DcuS resides in DcuS, and that DctA serves as an activity switch.
    Title: Differentiation of DctA and DcuS function in the DctA/DcuS sensor complex of Escherichia coli: function of DctA as an activity switch and of DcuS as the C4-dicarboxylate sensor.
  5. Reorganizing the DcuS homodimer and its interaction with DctA activate the kinase.
    Title: The cytoplasmic PASC domain of the sensor kinase DcuS of Escherichia coli: role in signal transduction, dimer formation, and DctA interaction.
  6. It is concluded that DctA forms a functional unit or sensor complex with DcuS through specific interaction sites.
    Title: Interaction of the Escherichia coli transporter DctA with the sensor kinase DcuS: presence of functional DctA/DcuS sensor units.
Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables L-aspartate transmembrane transporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables fumarate transmembrane transporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables fumarate transmembrane transporter activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables malate:proton symporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables malate:proton symporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables succinate transmembrane transporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables succinate transmembrane transporter activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables symporter activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within DNA damage response IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in L-aspartate transmembrane transport IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within L-aspartate transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in carboxylic acid transport IEA
Inferred from Electronic Annotation
more info
  
involved_in dicarboxylic acid transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within fumarate transport IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within fumarate transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in malate transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in succinate transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
C4 dicarboxylate/orotate:H(+) symporter

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3682161..3683447 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417985.1 C4 dicarboxylate/orotate:H(+) symporter [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417985.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376MKU7, A0A5B9ACJ5
    Conserved Domains (1) summary
    PRK01663
    Location:1 → 427
    PRK01663; C4-dicarboxylate transporter DctA; Reviewed
Nucleotide Protein
Heading Accession and Version

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Other Literature Sources
Molecular Biology Databases