waaC ADP-heptose:LPS heptosyltransferase 1 [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: waaC
Gene description: ADP-heptose:LPS heptosyltransferase 1
Primary source: ASAP:ABE-0011847
Locus tag: b3621
See related: ECOCYC:EG11189
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3611; rfa-2; rfaC; yibC
Summary: Overexpression causes abnormal biofilm architecture. [More information is available at EcoGene: EG11189]. The lipopolysaccharide of Escherichia coli K-12 consists of two major components: the hydrophobic lipid A moiety inserted into the outer membrane and the phosphorylated core oligosaccharide . [More information is available at EcoCyc: EG11189].
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Genomic context

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Sequence:: NC_000913.3 (3795979..3796938)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HepI enzyme undergoing a substrate binding-induced transition from an open to closed state prior to catalysis.
Title: Escherichia coli heptosyltransferase I: investigation of protein dynamics of a GT-B structural enzyme.
Among the nine mutants resistant to coliphage infection that authors selected, six were in the fhuA gene, two were mutated in the waaC gene, and one was mutated in the gmhD gene.
Title: A novel strategy to isolate cell-envelope mutants resistant to phage infection: bacteriophage mEp213 requires lipopolysaccharides in addition to FhuA to enter Escherichia coli K-12.
catalytic efficiency of Heptosyltransferase I with the fully deacylated analogue of Escherichia coli HepI LipidA
Title: Lipopolysaccharide biosynthesis without the lipids: recognition promiscuity of Escherichia coli heptosyltransferase I.
WaaC had access to the substrate before WaaA transfer was completed.
Title: Kdo-(2 --> 8)-Kdo-(2 --> 4)-Kdo but not Kdo-(2 --> 4)-Kdo-(2 --> 4)-Kdo is an acceptor for transfer of L-glycero-alpha-D-manno-heptose by Escherichia coli heptosyltransferase I (WaaC).
N-terminus verified by Edman degradation on complete protein
Title: Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC) gene, a gene required for lipopolysaccharide inner core synthesis.
Results report the structure of WaaC alone at 1.9 A resolution, and in complex with either ADP or the non-cleavable analog ADP-2-deoxy-2-fluoro-heptose of the sugar donor at 2.4 A resolution.
Title: Structure of the Escherichia coli heptosyltransferase WaaC: binary complexes with ADP and ADP-2-deoxy-2-fluoro heptose.

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Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ADP-heptose-lipopolysaccharide heptosyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glycosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables lipopolysaccharide heptosyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables lipopolysaccharide heptosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in lipopolysaccharide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in lipopolysaccharide core region biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipopolysaccharide core region biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipopolysaccharide core region biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipopolysaccharide core region biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ADP-heptose:LPS heptosyltransferase 1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3795979..3796938

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_418078.1 ADP-heptose:LPS heptosyltransferase 1 [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_418078.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A485J7M5

Conserved Domains (1) summary

PRK10964
Location:1 → 319

PRK10964; lipopolysaccharide heptosyltransferase RfaC